PDL2 Antibody [7C7] Cat. No.: RF16023

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Human
IMMUNOGEN: PD-L2 antibody was raised against the extracellular domain of human PD-L2.
TESTED APPLICATIONS: ELISA, Flow, ICC, IF, IHC-P, WB
APPLICATIONS: PD-L2 antibody can be used for detection of PD-L2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1207 - Raji Cell Lysate
 2) Cat. No. 1315 - Human Tonsil Tissue
PREDICTED MOLECULAR WEIGHT: Predicted: 30 kDa

Observed: 38 kDa

psi-iconProperties
PURIFICATION:PD-L2 Antibody is supplied as protein A purified IgG1.
CLONALITY:Monoclonal
ISOTYPE:IgG1
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PD-L2 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:PD-L2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:PDCD1LG2
ALTERNATE NAMES:PD-L2 Antibody: B7DC, Btdc, PDL2, CD273, PD-L2, PDCD1L2, bA574F11.2, B7DC, Programmed cell death 1 ligand 2, Butyrophilin B7-DC, PD-1 ligand 2
ACCESSION NO.:NP_079515
PROTEIN GI NO.:190014605
GENE ID:80380
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:PD-L2 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by co gnate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PD-L2 can act to stimulate an immunogenic response through and alternative receptor from PD-1.
REFERENCES:1) Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
2) Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
3) LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.
4) Zhang Y, Chung Y, Bishop C, et al. Regulation of T cell activation and tolerance by PDL2. Proc. Natl. Acad. Sci. USA 2006; 103:11695-700.

ANTIBODIES FOR RESEARCH USE ONLY.

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