Specifications
| HOST SPECIES: | Llama |
| SPECIES REACTIVITY: |
Human |
| IMMUNOGEN: |
PD-L1 antibody was raised against a recombinant protein corresponding to amino acids 19 - 239 of human PD-L1. |
| TESTED APPLICATIONS: |
Flow, ICC |
| APPLICATIONS: |
PD-L1 antibody can be used for detection of PD-L1 by Flow Cytometry and Immunocytochemistry at 10 μg/ml. |
| SPECIFICITY: |
At least three isoforms of PD-L1 are known to exist. |
Properties
| PURIFICATION: | PD-L1 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection. |
| CLONALITY: | Monoclonal |
| ISOTYPE: | sdAb |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | PD-L1 Antibody is supplied in PBS. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | PD-L1 antibody should be stored in working aliquots at -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Additional Info
| OFFICIAL SYMBOL: | CD274 |
| ALTERNATE NAMES: | PD-L1 Antibody: B7-H, B7H1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1, B7 homolog 1 |
| ACCESSION NO.: | NP_054862 |
| PROTEIN GI NO.: | 7661534 |
| GENE ID: | 29126 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
Background and References
| BACKGROUND: | PD-L1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. |
| REFERENCES: | 1) Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90. |
| 2) Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95. | | 3) LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78. |
ANTIBODIES FOR RESEARCH USE ONLY.
For additional information, visit ProSci's Terms & Conditions Page.
