This recombinant protein can be used for WB. For research use only.
Properties
PURITY:
>95% as determined by SDS-PAGE.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
PREDICTED MOLECULAR WEIGHT:
42.6 kDa
PHYSICAL STATE:
Lyophilized
BUFFER:
Tris with Glycine, Arginine and NaCl, pH7.5
STORAGE CONDITIONS:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Additional Info
ALTERNATE NAMES:
PDCD1,PD1,CD279,SLEB2
ACCESSION NO.:
G1SUF0
OFFICIAL SYMBOL:
PD-1
GENE ID:
100343779
Background and References
BACKGROUND:
Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-gammaby suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-gammasecretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
REFERENCES:
1) Ishida Y., et al., 1992, EMBO J. 11 (11): 3887–95.
2) Blank C., et al., 2007, Cancer Immunol. Immunother. 56 (5): 739–45.
Specifications
