This recombinant protein can be used for E, WB. For research use only.
BIOLOGICAL ACTIVITY:
Measured by its binding ability in a functional ELISA. Immobilized Human LDL R (High Purity) Protein, Strep Tag at 5 ug/mL (100 uL/well) can bind Hamster PCSK9 Protein, His Tag with a linear range of 6-50 ng/mL (QC tested).
Properties
PURITY:
>95% as determined by SDS-PAGE.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
PREDICTED MOLECULAR WEIGHT:
73 kDa
PHYSICAL STATE:
Lyophilized
BUFFER:
PBS, pH7.4
STORAGE CONDITIONS:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Additional Info
ALTERNATE NAMES:
PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
ACCESSION NO.:
G3GTK5
OFFICIAL SYMBOL:
PCSK9
Background and References
BACKGROUND:
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
REFERENCES:
1) Seidah NG, et al., Proc. Natl. Acad. Sci. U.S.A. 100 (3): 928–33.
2) Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
3) Dubuc G. et al., 2004, Arterioscler. Thromb. Vasc. Biol. 24 (8): 1454–9.
Specifications
