PCSK9 Recombinant Protein Cat. No.: 96-818

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psi-iconSpecifications
SPECIES:Hamster
SOURCE SPECIES:HEK293 cells
SEQUENCE:Gln 30 - Ser 691
FUSION TAG:His Tag
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:This recombinant protein can be used for E, WB. For research use only.
BIOLOGICAL ACTIVITY: Measured by its binding ability in a functional ELISA. Immobilized Human LDL R (High Purity) Protein, Strep Tag at 5 ug/mL (100 uL/well) can bind Hamster PCSK9 Protein, His Tag with a linear range of 6-50 ng/mL (QC tested).
psi-iconProperties
PURITY:>95% as determined by SDS-PAGE.

Endotoxin level is less than 1.0 EU per ug by the LAL method.
PREDICTED MOLECULAR WEIGHT:73 kDa
PHYSICAL STATE:Lyophilized
BUFFER:PBS, pH7.4
STORAGE CONDITIONS:Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
psi-iconAdditional Info
ALTERNATE NAMES:PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
ACCESSION NO.:G3GTK5
OFFICIAL SYMBOL:PCSK9
psi-iconBackground and References
BACKGROUND:Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
REFERENCES:1) Seidah NG, et al., Proc. Natl. Acad. Sci. U.S.A. 100 (3): 928–33.
2) Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
3) Dubuc G. et al., 2004, Arterioscler. Thromb. Vasc. Biol. 24 (8): 1454–9.

FOR RESEARCH USE ONLY.

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