PARP-1 [ARTD1] E988K Mutant Control Recombinant Protein Cat. No.: 90-343

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psi-iconSpecifications
SPECIES:Human
SOURCE SPECIES:Sf21 cells
SEQUENCE:Human full-length inactive mutant E998K of PARP-1 is fused to a HA-tag and a His-tag.
FUSION TAG:His Tag
TESTED APPLICATIONS:
APPLICATIONS:This recombinant proteins is for research use only.
BIOLOGICAL ACTIVITY: 0.5% of wild type PARP-1.
psi-iconProperties
PURITY:>95% (SDS-PAGE)
PHYSICAL STATE:Liquid
BUFFER:In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride and 50mM imidazole, 0.2% NP-40 and 10% glycerol.
CONCENTRATION:Lot dependent (0.2-0.5mg/ml)
STORAGE CONDITIONS:Stable for at least 6 months after receipt when stored at -80˚C.
psi-iconAdditional Info
ALTERNATE NAMES:Poly(ADP-ribose) Polymerase 1, ADP-ribosyltransferase Diphtheria Toxin-like 1, NAD(+) ADP-ribosyltransferase 1
ACCESSION NO.:P09874
PROTEIN GI NO.:156523968
OFFICIAL SYMBOL:PARP11
GENE ID:57097
psi-iconBackground and References
BACKGROUND:PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. PARP-1 (E998K mutant) is an inactive form of PARP-1 which can be used as a control compound.

FOR RESEARCH USE ONLY.

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