PAK2 Antibody Cat. No.: 3887

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rabbit: (93%)
IMMUNOGEN: Anti-PAK2 antibody (3887) was raised against a peptide corresponding to 14 amino acids near the amino terminus of human PAK2.

The immunogen is located within amino acids 20-70 of PAK2.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 0.5-2 μg/mL; IHC: 10 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry and Immunofluorescence in mouse samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1205 - Jurkat Cell Lysate
 2) Cat. No. 1406 - Mouse Spleen Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 58kD

Observed: 58 kD
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar PAK2 expression profile in human, mouse and rat cell lines detected by two independent anti-PAK2 antibodies that recognize different epitopes, 3885 against C-terminus domain and 3887 against N-terminus domain.  PAK2 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.

ISOFORMS: Human PAK2 has only one isoform (524aa, 58kD). Mouse PAK2 has one isoform (524aa, 58kD) and rat PAK2 also has one isoform (524aa, 58kD). 3887 can detect the isoforms of human, mouse and rat.

psi-iconProperties
PURIFICATION:PAK2 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PAK2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:PAK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:PAK2
ALTERNATE NAMES:PAK2 Antibody: PAK65, PAKgammaGamma-PAK, PAK-2
ACCESSION NO.:NP_002568
PROTEIN GI NO.:32483399
GENE ID:5062
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:PAK2 Antibody: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6, and are not activated by Cdc42/Rac binding. Of the six PAK proteins, only PAK2 is ubiquitously expressed and cleaved by caspase-3. This cleavage removes the amino-terminal regulatory domain and generates a constitutively active kinase fragment. Recent experiments have shown that following cleavage, the active fragment is myristoylated and directed to the plasma membrane and membrane ruffles where it promotes cell death via increased signaling through the c-Jun N-terminal kinase pathway, but without compromising mitochondrial integrity.
REFERENCES:1) Jaffer and Chernoff. Int. J. Biochem. Cell Biol. 2002; 34:713-7.
2) Rudel and Bokoch. Science 1997; 276:1571-4.
3) Vilas, et al. Proc. Natl. Acad. Sci. USA 2006; 103:6542-7.

ANTIBODIES FOR RESEARCH USE ONLY.

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