PACS1 Antibody Cat. No.: 8187

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: PACS1 antibody was raised against a 17 amino acid peptide near the center of human PACS1.

The immunogen is located within amino acids 430 - 480 of PACS1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: PACS1 antibody can be used for detection of PACS1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2 μg/mL For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
SPECIFICITY: PACS1 antibody is human, mouse and rat reactive. Multiple isoforms of PACS1 are known to exist. PACS1 antibody is predicted to not cross-react with PACS2.
POSITIVE CONTROL:1) Cat. No. 1478 - Rat Bladder Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 106 kDa

Observed: 105 kDa

psi-iconProperties
PURIFICATION:PACS1 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PACS1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:PACS1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:PACS1
ALTERNATE NAMES:Phosphofurin acidic cluster sorting protein 1, PACS-1, MRD17
ACCESSION NO.:NP_060496
PROTEIN GI NO.:30089916
GENE ID:55690
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The phosphofurin acidic cluster sorting protein 1 (PACS1), belongs to the PACS family of cytosolic connector proteins that localize different membrane proteins to the trans-Golgi network (TGN) (1,2). Mutations in PACS1 cause defects in cranial-neural crest migration during early development, leading to a recognizable intellectual disability syndrome (3). PACS1, along with the related protein PACS2, also plays a role in HIV-1 Nef-mediated downregulation of cell surface MHC-I molecules to the TGN, thereby enabling HIV-1 to escape immune surveillance (4).
REFERENCES:1) Wan L, Molloy SS, Thomas L, et al. PACS-1 defines a novel gene family of cytosolic sorting proteins required for trans-Golgi network localization. Cell 1998; 94:205-16.
2) Kottgen M, Benzing T, Simmen T, et al. Trafficking of TRPP2 by PACS proteins represents a novel mechanism of ion channel regulation. EMBO J. 2005; 24:705-16.
3) Schuurs-Hoeijmakers JH, Oh EC, Vissers LE, et al. Recurrent de novo mutations in PACS1 cause defective cranial-neural-crest migration and define a recognizable intellectual-disability syndrome. Am. J. Hum. Genet. 2012; 91:1122-7.
4) Dikeakos JD, Thomas L, Kwon G, et al. An interdomain binding site on HIV-1 Nef interacts with PACS-1 and PACS2 on endosomes to down-regulate MHC-I. Mol. Biol. Cell 2012; 23:2184-97.

ANTIBODIES FOR RESEARCH USE ONLY.

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