p53 (phospho Ser392) Antibody Cat. No.: XPS-1021

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Monkey
IMMUNOGEN: p53 (Ser392) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser392 of p53.
TESTED APPLICATIONS: WB
APPLICATIONS: Immunolabeling of the 53 kDa p53 protein in Western blots of PMA treated NIH3T3 cells is blocked by the Ser392 phosphopeptide used as antigen but by the corresponding non-phosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Immunohistochemistry: not determined. Human and some non-human primates have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
SPECIFICITY: p53 antibody is specific for the 53 kDa p53 protein phosphorylated at Ser392.
PREDICTED MOLECULAR WEIGHT: 53

psi-iconProperties
PURIFICATION:Affinity Purified
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
STORAGE CONDITIONS:For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:TP53
ALTERNATE NAMES:P53, BCC7, LFS1, TRP53, P53, Antigen NY-CO-13,
ACCESSION NO.:P04637
PROTEIN GI NO.:269849759
GENE ID:7157
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:p53 has a well established role in the blocking the proliferative action of damaged cells and acting in essence as an anticancer agent. It has been called the guardian of the genome. Phosphorylation of Ser392 in p53 is associated with formation of human tumors. In addition p53 has also been linked to affects of aging and oxidative stress. An increase in p53 has also been linked to deficits in LTP and learning and memory. We raised this polyclonal antibody against a peptide representing the sequence around Ser392 of p53.
REFERENCES:1) Saito, S., Yamaguchi, H., Higashimoto, Y., Chao, C., Xu, Y., Fornace, A.J., Jr., Appella, E., and Anderson, C.W., "Phosphorylation site interdependence of human p53 post-translational modifications in response to stress," J. Biol. Chem., 278 (2003) 37,536-37,544.
2) Sharpless,N.E. and DePinho,R.A., "p53: Good cop/bad cop," Cell, 110 (2002) 9-12.
3) Jiang, Y.H., Armstrong, D., Albrecht, U., Atkins, C.M., Noebels, J.L., Eichele, G., Sweatt, J.D., and Beaudet, A.L., "Mutation of the angelman ubiquitin ligase in mice causes increased cytoplasmic p53 and deficits of contextual learning and long-term potentiation," Neuron, 21 (1998) 799 - 811.
4) Pise-Masison, C.A., Radonovich, M., Sakaguchi, K., Appella, E., and Brady, J.N., "Phosphorylation of p53: a novel pathway for p53 inactivation in human T- cell lymphotropic virus type 1-transformed cells," J. Virol., 72 (1998) 6348-6355.

ANTIBODIES FOR RESEARCH USE ONLY.

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