P2RX7 Antibody Cat. No.: 8015

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (81%)
IMMUNOGEN: P2RX7 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human P2RX7.

The immunogen is located within the last 50 amino acids of P2RX7.
TESTED APPLICATIONS: ELISA, ICC, IF, WB
APPLICATIONS: P2RX7 antibody can be used for detection of P2RX7 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPECIFICITY: P2RX7 antibody is human, mouse, and rat reactive. Multiple isoforms of P2RX7 are known to exist.
POSITIVE CONTROL:1) Cat. No. 1212 - 3T3 Cell Lysate
 2) Cat. No. 17-201 - 3T3/BALB Cell Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 34, 47, 65 kDa

Observed: 54 kDa

psi-iconProperties
PURIFICATION:P2RX7 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:P2RX7 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:P2RX7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:P2RX7
ALTERNATE NAMES:P2X purinoceptor 7, P2X7, purinergic receptor P2X ligand-gated ion channel 7
ACCESSION NO.:NP_002553
PROTEIN GI NO.:300068987
GENE ID:5027
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The purinergic receptor P2X ligand-gated ion channel 7 (P2RX7) belongs to the family of purinoceptors for ATP (1). This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules (1,2). Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression (2). Recent studies have suggested that P2RX7 may play a key role in immune-mediated diseases such as rheumatoid arthritis (3) as well as neuropsychiatric disorders (4).
REFERENCES:1) Surprenant A, Rassendren F, Kawashima E, et al. The cytosolic P2Z receptor for extracellular ATP identified as a P2X receptor (P2X7). Science 1996; 272:735-8.
2) North RA. Molecular physiology of P2X receptors. Physiol. Rev. 2002; 82:1013-67.
3) Labasi JM, Petrushova N, Donovan C, et al. Absence of the P2X7 receptor alters leukocyte function and attenuates an inflammatory response. J. Immunol. 168:6436-45.
4) Basso AM, Bratcher NA, Harris RR, et al. Behavioral profile of P2X7 receptor knockout mice in animal models of depression and anxiety; relevance for neuropsychiatric disorders. Behav. Brain Res. 2009; 198:83-90.

ANTIBODIES FOR RESEARCH USE ONLY.

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