NSA1 Antibody Cat. No.: 5671

Availability: In stock

$0.00

 Price Options

* Required Fields

$0.00
psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (79%)
IMMUNOGEN: NSA1 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human NSA1.

The immunogen is located within amino acids 90 - 140 of NSA1.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: NSA1 antibody can be used for detection of NSA1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1464 - Rat Liver Tissue Lysate

psi-iconProperties
PURIFICATION:NSA1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:NSA1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:NSA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:WDR74
ALTERNATE NAMES:NSA1 Antibody: NSA1, WD repeat-containing protein 74, NOP seven-associated protein 1
ACCESSION NO.:Q6RFH5
PROTEIN GI NO.:55976441
GENE ID:54663
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:NSA1 Antibody: The yeast nucleolar protein NOP7 is necessary for the maturation of 66S preribosomes and interacts with numerous other proteins. One such protein is an essential, conserved WD repeat protein, NOP seven-associated protein 1 (NSA1), that is also required for the yeast 66S ribosome assembly. NSA1 is also associated with the AAA ATPase Rix7, and release of NSA1 from a novel late nucleolar pre-60S requires the Rix7 function. NSA1 has also been found upregulated in mammalian cancer cells, suggesting it may also play a role in cell proliferation.
REFERENCES:1) Miles TD, Jakovljevic J, Horsey EW, et al. Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes. Mol. Cell Biol.2005; 25:10419-32.
2) Harnpicharnchai P, Jakovljevic J, Horsey E, et al. Composition and functional characterization of yeast 66S ribosome assembly intermediates. Mol. Cell2001; 8:505-15.
3) Kressler D, Roser D, Pertschy B, et al. The AAA ATPase Rix7 powers progression of ribosome biogenesis by stripping Nsa1 from pre-60S particles. J. Cell Biol.2008; 181:935-44.
4) Krol M, Polanska J, Pawlowski KM, et al. Transcriptomic signature of cell lines isolated from canine mammary adenocarcinoma metastases to lungs. J. Appl. Genet.2010; 51:37-50.

ANTIBODIES FOR RESEARCH USE ONLY.

For additional information, visit ProSci's Terms & Conditions Page.