Datasheet

NO66 Antibody
CATALOG NUMBER: 46-636

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:H
TESTED APPLICATIONS:E, WB
APPLICATIONS:ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 70 kDa band observed in lysates of cell line A431 (calculated MW of 71.1 kDa according to NP_078920.2). Recommended concentration: 0.3-1 μg/mL.
POSITIVE CONTROL:1) Cat. No. 1202-A431 Cell Lysate
IMMUNOGEN:NO66 antibody was raised against an 11 amino acid synthetic peptide near the internal region of NO66.
HOST SPECIES:Goat

Properties

PURIFICATION:NO66 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PHYSICAL STATE:Liquid
BUFFER:NO66 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
CONCENTRATION:500 ug/mL
STORAGE CONDITIONS:Aliquot and store at-20˚C. Minimize freezing and thawing.
CLONALITY:This is a polyclonal antibody.
CONJUGATE:None

Additional Info

ALTERNATE NAMES:C14orf169, chromosome 14 open reading frame 169, FLJ21802, NO66, MAPJD, C14orf169
ACCESSION NO.:NP_078920.2
PROTEIN GI NO.:106879206

Background

REFERENCES
1) Eilbracht J, Reichenzeller M, Hergt M, Schnolzer M, Heid H, Stohr M, Franke WW, Schmidt-Zachmann MS. NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin. Mol Biol Cell. 2004 Apr;15(4):1816-32. Epub 2004 Jan 23.

For Research Use Only