NMDA NR1 Antibody Cat. No.: XPS-2023

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Rat
IMMUNOGEN: NMDA NR1, Splice Variant C2’ polyclonal antibody was raised against a peptide containing the C2’ insert.
TESTED APPLICATIONS: IHC, WB
APPLICATIONS: NMDA NR1, Splice Variant C2' antibody allows for strong and specific immunolabeling of the NR1 subunit containing the C2’ splice variant insert. Rabbit anti-NMDA NR1, Splice Variant C2’ does not recognize NR1 subunits that do not contain the C2’ insert. Applications include Dot Blots (DB), Immunohistochemistry (IHC) and Western Blots (WB). Rabbit anti-NMDA NR1, Splice Variant C2 recognizes NR1 in rat and mouse brain. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, DB and WB.
PREDICTED MOLECULAR WEIGHT: 120

psi-iconProperties
PURIFICATION:Affinity Purified
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Lyophilized
STORAGE CONDITIONS:NMDA NR1, Splice Variant C2' antibody can be stored at -20˚C. After reconstitution in 50 μl PBS, the antibody should be aliquot and stored at -20˚C and is stable at -20˚C for at least 1 year. Ambient shipment is suitable for both international and domestic.

psi-iconAdditional Info
OFFICIAL SYMBOL:Grin1
ALTERNATE NAMES:NR1, GluN1, NMDAR1, Nmdar1, N-methyl-D-aspartate receptor subunit NR1,
ACCESSION NO.:P35439
PROTEIN GI NO.:548379
GENE ID:24408
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s disease, epilepsy and ischemic neuronal cell death. Increased membrane surface expression of the NR1 subunit of the receptor has been associated with synaptic plasticity. There are a number of different splice variants of the NR1. Differential splicing of three exons in the NR1 subunit generates up to eight NR1 splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.
REFERENCES:1) Grosshans, D.R., Clayton, D.A., Coultrap, S.J., and Browning, M.D., "LTP leads to rapid surface expression on NMDA not AMPA receptors in adult rat CA1," Nature Neurosci., 5 (2002) 27 - 33.
2) Luo, J.H., Wang, Y.H., Yasuda, R.P., Dunah, A.W. and Wolfe, B.B., "The majority of N-methyl-D-aspartate receptor complexes in adult rat cerebral cortex contain at least three different subunits (NR1/NR2A/NR2B)," Mol. Pharmacol. 51 (1997) 79 - 86.
3) Zukin, R.S. and Bennett, M.V.L., "Alternatively spliced isoforms of the NMDARI receptor subunit," Trends Neurosci., 18 (1995) 306 - 313.
4) Laurie, D.J. and Seeburg, P.H., "Regional and developmental heterogeneity in splicing of the rat brain NMDAR1 mRNA," J. Neurosci., 14 (1994) 3180 - 3194.

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