Datasheet

NIPSNAP3A Antibody
CATALOG NUMBER: 4947

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:NIPSNAP3A antibody can be used for detection of NIPSNAP3A by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1403 - Mouse Brain Tissue Lysate
SPECIFICITY:At least two isoforms of NIPSNAP3A are known to exist. NIPSNAP3A antibody is predicted to not cross-react with any other members of the NIPSNAP protein family.
IMMUNOGEN:NIPSNAP3A antibody was raised against a 16 amino acid synthetic peptide near the center of human NIPSNAP3A.

The immunogen is located within amino acids 40 - 90 of NIPSNAP3A.
HOST SPECIES:Rabbit

Properties

PURIFICATION:NIPSNAP3A Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:NIPSNAP3A Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:NIPSNAP3A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:NIPSNAP3A Antibody: TASSC, HSPC299, NIPSNAP4, Protein NipSnap homolog 3A, Protein NipSnap homolog 4, NipSnap3A
ACCESSION NO.:NP_056284
PROTEIN GI NO.:22267436
OFFICIAL SYMBOL:NIPSNAP3A
GENE ID:25934

Background

BACKGROUND:NIPSNAP3A Antibody: The NIPSNAP proteins comprise a family of evolutionarily well-conserved proteins with strong sequence similarity to the central portion of a protein encoded by C. elegans chromosome III between a 4-nitrophenylphosphatase (NIP) domain and non-neuronal SNAP25-like protein. NIPSNAP2, a novel gene encoding a protein with tyrosine phosphorylation sites and a transmembrane domain, is co-amplified with EGFR in approximately 40% of glioblastomas, the most common and malignant form of central nervous system tumors. While NIPSNAP3B is highly expressed skeletal muscle, NIPSNAP3A mRNA levels are low. NIPSNAP3A protein is associated with plasma membrane and partially localized in rafts. NIPSNAP proteins have been suggested to be important in vesicular transport.
REFERENCES: 1) Seroussi E, Pan HQ, Kedra D, et al. Characterization of the human NIPSNAP1 gene from 22q12: a member of a novel gene family. Gene1998; 212:13-20.
2) Wang X-Y, Smith DI, Liu W, et al. GBAS, a novel gene encoding a protein with tyrosine phosphorylation sites and a transmembrane domain, is co-amplified with EGFR. Genomics1998; 49:448-51.
3) Buechler C, Bodzioch M, Bared SM, et al. Expression pattern and raft association of NIPSNAP3 and NIPSNAP4, highly homologous proteins encoded by genes in close proximity to the ATP-binding cassette transporter A1. Genomics2004; 83:1116-24.

For Research Use Only