NINJ1 Antibody Cat. No.: 7967

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: NINJ1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human NINJ1.

The immunogen is located within the last 50 amino acids of NINJ1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: NINJ1 antibody can be used for detection of NINJ1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPECIFICITY: NINJ1 antibody is human, mouse and rat reactive.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 17 kDa

Observed: 16 kDa

psi-iconProperties
PURIFICATION:NINJ1 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:NINJ1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:NINJ1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:NINJ1
ALTERNATE NAMES:NINJ1 Antibody: NIN1, NINJURIN, Ninjurin-1, Nerve injury-induced protein 1
ACCESSION NO.:NP_004139
PROTEIN GI NO.:148922911
GENE ID:4814
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Ninjurin-1 (NINJ1) is a homophilic adhesion molecule that is strongly induced in dorsal root ganglion (DRG) and Schwann cells following nerve injury and promotes neurite outgrowth of DRG neurons (1,2), suggesting that NINJ1 may play a role in nerve regeneration. NINJ1 is transcriptionally regulated by p53 and is induced by DNA damage in a p53-dependent manner. A deficiency of NINJ1 increases p53 expression, suppresses cell proliferation, but enhances apoptosis and premature senescence in a p53-dependent manner as well as radiation-induced cell mortality (3).
REFERENCES:1) Araki T and Milbrandt J. Ninjurin, a novel adhesion molecule, is induced by nerve injury and promotes axonal growth. Neuron 1996; 17:353-61.
2) Araki T, Zimonjic DB, Popescu NC, et al. Mechanism of homophilic binding mediated by ninjurin, a novel widely expressed adhesion molecule. J. Biol. Chem. 1997; 212:21373-80.
3) Cho SJ, Rossi A, Jung YS, et al. Ninjurin1, a target of p53, regulates p53 expression and p53-dependent cell survival, sensecence, and radiation-induced mortality. Proc. Natl. Acad. Sci. USA 2013; 110:9362-7.

ANTIBODIES FOR RESEARCH USE ONLY.

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