Datasheet

NINJ1 Antibody
CATALOG NUMBER: 7967

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:NINJ1 antibody can be used for detection of NINJ1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 17 kDa

Observed: 16 kDa
SPECIFICITY:NINJ1 antibody is human, mouse and rat reactive.
IMMUNOGEN:NINJ1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human NINJ1.

The immunogen is located within the last 50 amino acids of NINJ1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:NINJ1 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:NINJ1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:NINJ1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:NINJ1 Antibody: NIN1, NINJURIN, Ninjurin-1, Nerve injury-induced protein 1
ACCESSION NO.:NP_004139
PROTEIN GI NO.:148922911
OFFICIAL SYMBOL:NINJ1
GENE ID:4814

Background

BACKGROUND:Ninjurin-1 (NINJ1) is a homophilic adhesion molecule that is strongly induced in dorsal root ganglion (DRG) and Schwann cells following nerve injury and promotes neurite outgrowth of DRG neurons (1,2), suggesting that NINJ1 may play a role in nerve regeneration. NINJ1 is transcriptionally regulated by p53 and is induced by DNA damage in a p53-dependent manner. A deficiency of NINJ1 increases p53 expression, suppresses cell proliferation, but enhances apoptosis and premature senescence in a p53-dependent manner as well as radiation-induced cell mortality (3).
REFERENCES: 1) Araki T and Milbrandt J. Ninjurin, a novel adhesion molecule, is induced by nerve injury and promotes axonal growth. Neuron 1996; 17:353-61.
2) Araki T, Zimonjic DB, Popescu NC, et al. Mechanism of homophilic binding mediated by ninjurin, a novel widely expressed adhesion molecule. J. Biol. Chem. 1997; 212:21373-80.
3) Cho SJ, Rossi A, Jung YS, et al. Ninjurin1, a target of p53, regulates p53 expression and p53-dependent cell survival, sensecence, and radiation-induced mortality. Proc. Natl. Acad. Sci. USA 2013; 110:9362-7.

For Research Use Only