NIBRIN Antibody Cat. No.: 6841

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Chicken: (85%)
IMMUNOGEN: NIBRIN antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human NIBRIN.

The immunogen is located within amino acids 50 - 100 of NIBRIN.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: NIBRIN antibody can be used for detection of NIBRIN by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: At least three alternatively spliced transcript isoforms of NIBRIN are known to exist.
POSITIVE CONTROL:1) Cat. No. 1462 - Rat Lung Tissue Lysate
 2) Cat. No. 10-101 - Human Lung Tissue Slide

psi-iconProperties
PURIFICATION:NIBRIN Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:NIBRIN Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:NIBRIN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:NBN
ALTERNATE NAMES:NIBRIN Antibody: ATV, NBS, P95, NBS1, AT-V1, AT-V2, Nibrin, Cell cycle regulatory protein p95
ACCESSION NO.:NP_002476
PROTEIN GI NO.:33356172
GENE ID:4683
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:NIBRIN Antibody: NIBRIN (NBN) is a member of the double-strand break repair complex MRE11/RAD50/NBN (MRN) which is involved in DNA double-strand break repair, DNA damage-induced checkpoint activation and plays a critical role in the maintenance of chromosome integrity. NIBRIN contains two modules found in cell cycle checkpoint proteins, a forkhead-associated domain adjacent to a breast cancer carboxy-terminal domain. Mutations in this gene are associated with Nijmegen breakage syndrome and maybe the cause of cancer predisposition and aplastic anemia.
REFERENCES:1) Carney JP, Maser RS, Olivares H, et al. The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response. Cell 1998; 93:477-86
2) Marcelain K, De La Torre C, Gonzalez P, et al. Roles of nibrin and AtM/ATR kinases on the G2 checkpoint under endogenous or radio-induced DNA damage. Biol. Res. 2005; 38:179-85.
3) Varon R, Vissinga C, Platzer M, et al. Nibrin, a novel DNA double-strand break repair protein, is mutated in Nijmegen breakage syndrome. Cell 1998; 93:467-76.
4) Heikkinen K, Karppinen SM, Soini Y. et al. Mutation screening of Mre11 complex genes: indication of RAD50 involvement in breast and ovarian cancer susceptibility. J. Med. Genet. 2003; 40:E131.

ANTIBODIES FOR RESEARCH USE ONLY.

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