NGN2 Antibody Cat. No.: 7091

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Rabbit polyclonal NGN2 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human NGN2.

The immunogen is located within the last 50 amino acids of NGN2.
TESTED APPLICATIONS: ELISA, IF, WB
APPLICATIONS: NGN2 antibody can be used for detection of NGN2 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: NGN2 antibody is predicted to not cross-react with neurogenin 1.
POSITIVE CONTROL:1) Cat. No. 1408 - Mouse Small Intestine Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
PREDICTED MOLECULAR WEIGHT: 30 kDa

psi-iconProperties
PURIFICATION:NGN2 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:NGN2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:NGN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:NEUROG2
ALTERNATE NAMES:NGN2 Antibody: NGN2, Atoh4, ngn-2, Math4A, bHLHa8, ATOH4, BHLHA8, NGN2, Neurogenin-2, Class A basic helix-loop-helix protein 8, NGN-2
ACCESSION NO.:NP_076924
PROTEIN GI NO.:31077092
GENE ID:63973
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:NGN2 Antibody: Neurogenin-2 (NGN2) is a neural-specific basic helix-loop-helix (bHLH) transcription factor that can specify a neuronal fate on ectodermal cells and is expressed in neural progenitor cells within the developing central and peripheral nervous systems. NGN2 is thought to work with Nurr1 to play a role in the differentiation and survival of midbrain dopaminergic neurons. It has also been suggested for use in human embryonic neural progenitors as a graft for spinal cord injuries
REFERENCES:1) Sommer L, Ma Q, and Anderson DJ. Neurogenins, a novel family of atonal-related bHLH transcription factors, a putative mammalian neuronal determination genes that reveal progenitor cell heterogeneity in the developing CNS and PNS. Mol. Cell Neurosci. 1996; 8:221-41.
2) Fode C, Gradwohl G, Morin X, et al. The bHLH protein NEUROGENIN 2 is a determination factor for epibranchial placode-derived sensory neurons. Neuron 1998; 20:483-94.
3) Andersson EK, Irvin DK, Ahlsio J, et al. Ngn2 and Nurr1 act in synergy to induce midbrain dopaminergic neurons from expanded neural stem and progenitor cells. Exp. Cell. Res. 2007; 313:1172-80.
4) Perrin FE, Boniface G, Serguera C, et al. Grafted human embryonic progenitors expressing neurogenin-2 stimulate axonal sprouting and improve motor recovery after severe spinal cord injury. PLoS One 2010; 5:e15914.

ANTIBODIES FOR RESEARCH USE ONLY.

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