Datasheet

Neurotrypsin Antibody
CATALOG NUMBER: 4105

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:Neurotrypsin antibody can be used for detection of neurotrypsin by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 96 kDa

Observed: 96 kDa
IMMUNOGEN:Neurotrypsin antibody was raised against a 23 amino acid synthetic peptide from near the amino terminus of human neurotrypsin.

The immunogen is located within amino acids 110 - 160 of Neurotrypsin.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Neurotrypsin Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Neurotrypsin Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Neurotrypsin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Neurotrypsin Antibody: MRT1, BSSP3, BSSP-3, Neurotrypsin, Leydin
ACCESSION NO.:CAA04816
PROTEIN GI NO.:2661424
OFFICIAL SYMBOL:PRSS12
GENE ID:8492

Background

BACKGROUND:Neurotrypsin Antibody: Neurotrypsin is a central nervous system-expressed serine protease whose truncation or absence causes nonsyndromic mental retardation. It is most prominently expressed in structures that are involved in the processing and storage of learned behavior and memory, such as the cerebral cortex, the hippocampus, and amygdala. Evidence suggests that neurotrypsin has multiple functions, including axonal outgrowth, maintaining neuronal plasticity, and arranging the perineuronal environment, partly in coordination with other proteases including tissue plasminogen activator. At least two isoforms of neurotrypsin are known to exist.
REFERENCES: 1) Gschwend TP, Krueger SR, Kozlov SV, et al. Neurotrypsin, a novel multidomain serine protease expressed in the nervous system. Mol. Cell. Neurosci.1997; 9:207-19.
2) Molinari F, Rio M, Meskanaite V, et al. Truncating neurotrypsin mutation in autosomal recessive nonsyndromic mental retardation. Science2002; 1779-81.
3) Mitsui S, Yamaguchi N, Osako Y, et al. Enzymatic properties and localization of motopsin (PRSS12), a protease who absence causes mental retardation. Brain Res.2007; 1136:1-12.

For Research Use Only