| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse |
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rat: (100%) |
| IMMUNOGEN: | Neuritin antibody was raised against a 20 amino acid synthetic peptide from near the center of human neuritin. The immunogen is located within amino acids 70 - 120 of Neuritin. |
| TESTED APPLICATIONS: | ELISA, IF, IHC-P, WB |
| APPLICATIONS: | Neuritin antibody can be used for detection of Neuritin by Western blot at 5 and 10 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. |
| POSITIVE CONTROL: | 1) Cat. No. 1224 - Daudi Cell Lysate |
| 2) Cat. No. 1401 - Mouse Heart Tissue Lysate | |
| PREDICTED MOLECULAR WEIGHT: | Predicted: 16 kDa Observed: 15 kDa |
Specifications| PURIFICATION: | Neuritin Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | Neuritin Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | Neuritin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | NRN1 |
| ALTERNATE NAMES: | Neuritin Antibody: NRN, dJ380B8.2, NRN, Neuritin |
| ACCESSION NO.: | NP_057672 |
| PROTEIN GI NO.: | 7706123 |
| GENE ID: | 51299 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | Neuritin Antibody: As the nervous system of a complex organism develops, it establishes functional networks through the growth and retraction of synaptic connections from growing axons and dendrites. This synaptic remodeling involves neuro-transmitter signaling, activation of neurotrophin receptors and alterations in gene expression. One such gene whose expression is increased by neural activity is neuritin, a GPI-anchored protein that is expressed in postmitotic differentiating neurons of the developing nervous system. Its expression is also induced by the neurotrophins BDNF and NT-3. Purified recombinant neuritin promotes neurite outgrowth and arborization in primary embryonic neuronal cultures, suggesting that neuritin may play a role as a downstream effector of activity-induced neurite outgrowth. More recent experiments have shown that neuritin is required for the androgen-induced axonal elongation in motor neurons and is upregulated following spinal cord injury, suggesting that neuritin may also play a role in survival and axonal regeneration. |
| REFERENCES: | 1) Li Z and Sheng M. Some assembly required: the development of neuronal synapses. Nat. Rev. Mol. Cell Biol. 2003; 4:833-41. | 2) Naeve GS, Ramakrishnan M, Kramer R, et al. Neuritin: A gene induced by neural activity and neurotrophins that promotes neuritogenesis. Proc. Natl. Acad. Sci. USA 1997; 94:2648-53. | 3) Marron TU, Guerini V, Rusmini P, et al. Androgen-induced neurite outgrowth is mediated by neuritin in motor neurons. J. Neurochem. 2005; 92:10-20. | 4) Di Giovanni S, De Biase A, Yakovlev A, et al. In vivo and in vitro characterization of novel neuronal plasticity factors identified following spinal cord injury. J. Biol. Chem. 2005; 280:2084-91. |
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