Datasheet

Neuritin Antibody
CATALOG NUMBER: 4101

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rat: (100%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:Neuritin antibody can be used for detection of Neuritin by Western blot at 5 and 10 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1224 - Daudi Cell Lysate
 2) Cat. No. 1401 - Mouse Heart Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 16 kDa

Observed: 15 kDa
IMMUNOGEN:Neuritin antibody was raised against a 20 amino acid synthetic peptide from near the center of human neuritin.

The immunogen is located within amino acids 70 - 120 of Neuritin.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Neuritin Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Neuritin Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Neuritin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Neuritin Antibody: NRN, dJ380B8.2, NRN, Neuritin
ACCESSION NO.:NP_057672
PROTEIN GI NO.:7706123
OFFICIAL SYMBOL:NRN1
GENE ID:51299

Background

BACKGROUND:Neuritin Antibody: As the nervous system of a complex organism develops, it establishes functional networks through the growth and retraction of synaptic connections from growing axons and dendrites. This synaptic remodeling involves neuro-transmitter signaling, activation of neurotrophin receptors and alterations in gene expression. One such gene whose expression is increased by neural activity is neuritin, a GPI-anchored protein that is expressed in postmitotic differentiating neurons of the developing nervous system. Its expression is also induced by the neurotrophins BDNF and NT-3. Purified recombinant neuritin promotes neurite outgrowth and arborization in primary embryonic neuronal cultures, suggesting that neuritin may play a role as a downstream effector of activity-induced neurite outgrowth. More recent experiments have shown that neuritin is required for the androgen-induced axonal elongation in motor neurons and is upregulated following spinal cord injury, suggesting that neuritin may also play a role in survival and axonal regeneration.
REFERENCES: 1) Li Z and Sheng M. Some assembly required: the development of neuronal synapses. Nat. Rev. Mol. Cell Biol. 2003; 4:833-41.
2) Naeve GS, Ramakrishnan M, Kramer R, et al. Neuritin: A gene induced by neural activity and neurotrophins that promotes neuritogenesis. Proc. Natl. Acad. Sci. USA 1997; 94:2648-53.
3) Marron TU, Guerini V, Rusmini P, et al. Androgen-induced neurite outgrowth is mediated by neuritin in motor neurons. J. Neurochem. 2005; 92:10-20.
4) Di Giovanni S, De Biase A, Yakovlev A, et al. In vivo and in vitro characterization of novel neuronal plasticity factors identified following spinal cord injury. J. Biol. Chem. 2005; 280:2084-91.

For Research Use Only