NEUCRIN Antibody Cat. No.: 6493

Availability: In stock

$0.00

 Price Options

* Required Fields

$0.00
psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Chicken: (100%), Bovine: (100%)
IMMUNOGEN: Neucrin antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human Neucrin.

The immunogen is located within the last 50 amino acids of NEUCRIN.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: Neucrin antibody can be used for detection of Neucrin by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPECIFICITY: At least three isoforms of Neucrin are known to exist; this antibody will detect the two shorter isoforms. Neucrin antibody is predicted to not cross-react with RILP.
POSITIVE CONTROL:1) Cat. No. 1473 - Rat Cerebellum Tissue Lysate

psi-iconProperties
PURIFICATION:NEUCRIN Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:NEUCRIN Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:NEUCRIN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:DRAXIN
ALTERNATE NAMES:NEUCRIN Antibody: UNQ3119, neucrin, AGPA3119, C1orf187, PSEC0258, UNQ3119/PRO10268, Draxin, Dorsal inhibitory axon guidance protein
ACCESSION NO.:BAG80561
PROTEIN GI NO.:86990448
GENE ID:374946
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:NEUCRIN Antibody: Neucrin, also known as Draxin, is a repulsive guidance protein for the spinal cord and forebrain commissures. It is thought to act as an antagonist of canonical Wnt signaling by inhibiting the stabilization of cytosolic beta-catenin. Ectopically expressed neucrin inhibited growth or caused misrouting of chick spinal cord commissural axons in vivo while Neucrin-null mice showed defasciculation of spinal cord commissural axons and an absence of all forebrain commisures. Other experiments in mice have shown that olfactory bulb axonal outgrowth is inhibited by Neucrin, suggesting that Neucrin functions as an inhibitory guidance cue for olfactory bulb axons.
REFERENCES:1) Islam SM, Shinmyo Y, Okafuji T, et al. Draxin, a repulsive guidance protein for spinal cord and forebrain commissures. Science 2009; 323:388-93.
2) Miyake A, Takahashi Y, Miwa H, et al. Neucrin is a novel neural-specific secreted antagonist to canonical Wnt signaling. Biochem. Biophys. Res. Commun. 2009; 390:1051-5.
3) Ahmed G, Shinmyo Y, Naser IB, et al. Olfactory bulb axonal outgrowth is inhibited by draxin. Biochem. Biophys. Res. Commun. 2010; 398:730-4.

ANTIBODIES FOR RESEARCH USE ONLY.

For additional information, visit ProSci's Terms & Conditions Page.