Nephrin Antibody Cat. No.: 7979

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (93%), Rat: (93%)
IMMUNOGEN: Nephrin antibody was raised against a 15 amino acid peptide near the carboxy terminus of human Nephrin.

The immunogen is located within amino acids 1100 - 1150 of Nephrin.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: Nephrin antibody can be used for detection of Nephrin by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry at 5 μg/ml. For immunofluorescence start at 20 μg/mL

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: Nephrin antibody is human specific.
POSITIVE CONTROL:1) Cat. No. 1305 - Human Kidney Tissue Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 125, 137 kDa

Observed: 125 kDa

psi-iconProperties
PURIFICATION:Nephrin antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Nephrin antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Nephrin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:NPHS1
ALTERNATE NAMES:NPHN, NPHS1, Renal glomerulus-specific cell adhesion receptor3
ACCESSION NO.:NP_004637
PROTEIN GI NO.:4758822
GENE ID:4648
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth (1). Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm (2,3).
REFERENCES:1) Kestila M, Lenkkeri U, Mannikko M, et al. Positionally cloned gene for a novel glomerular protein – Nephrin – is mutated in congenital nephrotic syndrome. Mol. Cell 1998; 1:575-582.
2) Tryggvason K. Unraveling the mechanisms of glomerular ultrafiltration: nephrin, a key component of the slit diaphragm. J. Am. Soc. Nephrol. 1999; 10:2440-5.
3) Tryggvason K and Wartiovaara J. Molecular basis of glomerular permselectivity. Curr. Opin. Nephrol. Hypertens. 2001; 10:543-9.

ANTIBODIES FOR RESEARCH USE ONLY.

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