Datasheet

Nephrin Antibody
CATALOG NUMBER: 7979

Availability: In stock

$0.00
Product Price Options

* Required Fields

$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (93%), Rat: (93%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:Nephrin antibody can be used for detection of Nephrin by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry at 5 μg/ml. For immunofluorescence start at 20 μg/mL
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1305 - Human Kidney Tissue Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 125, 137 kDa

Observed: 125 kDa
SPECIFICITY:Nephrin antibody is human specific.
IMMUNOGEN:Nephrin antibody was raised against a 15 amino acid peptide near the carboxy terminus of human Nephrin.

The immunogen is located within amino acids 1100 - 1150 of Nephrin.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Nephrin antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Nephrin antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Nephrin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:NPHN, NPHS1, Renal glomerulus-specific cell adhesion receptor3
ACCESSION NO.:NP_004637
PROTEIN GI NO.:4758822
OFFICIAL SYMBOL:NPHS1
GENE ID:4648

Background

BACKGROUND:Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth (1). Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm (2,3).
REFERENCES: 1) Kestila M, Lenkkeri U, Mannikko M, et al. Positionally cloned gene for a novel glomerular protein – Nephrin – is mutated in congenital nephrotic syndrome. Mol. Cell 1998; 1:575-582.
2) Tryggvason K. Unraveling the mechanisms of glomerular ultrafiltration: nephrin, a key component of the slit diaphragm. J. Am. Soc. Nephrol. 1999; 10:2440-5.
3) Tryggvason K and Wartiovaara J. Molecular basis of glomerular permselectivity. Curr. Opin. Nephrol. Hypertens. 2001; 10:543-9.

For Research Use Only