Datasheet

NENF Antibody
CATALOG NUMBER: 4753

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:NENF antibody can be used for detection of NENF by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight of ~20kDa, NENF is observed at 37 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1308 - Human Small Intestine Tissue Lysate
IMMUNOGEN:NENF antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human NENF.

The immunogen is located within the last 50 amino acids of NENF.
HOST SPECIES:Rabbit

Properties

PURIFICATION:NENF Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:NENF Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:NENF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:NENF Antibody: CIR2, SPUF, SCIRP10, CIR2, Neudesin, Cell immortalization-related protein 2, SPUF protein
ACCESSION NO.:NP_037481
PROTEIN GI NO.:7019545
OFFICIAL SYMBOL:NENF
GENE ID:29937

Background

BACKGROUND:NENF Antibody: NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor.
REFERENCES: 1) Kimura I, Yoshioka M, Konishi M, et al. Neudesin, a novel secreted protein with a unique primary structure and neurotrophic activity. J. Neurosci. Res.2005; 79:287-94.

For Research Use Only