Datasheet

NDST2 Antibody
CATALOG NUMBER: 7869

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:NDST2 antibody can be used for detection of PDCL by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1288 - A20 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 97 kDa

Observed: 101 kDa
SPECIFICITY:NDST2 antibody is human, mouse and rat reactive. At least two isoforms of NDST2 are known to exist; this antibody will only detect the larger isoform. NDST2 antibody is predicted not to cross-react with other members of the NDST protein family.
IMMUNOGEN:NDST2 antibody was raised against a 16 amino acid peptide near the amino terminus of human NDST2.

The immunogen is located within amino acids 20 - 70 of NDST2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:NDST2 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:NDST2 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:NDST2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:NDST2 Antibody: NST2, HSST2, N-heparan sulfate sulfotransferase 2, NDST-2
ACCESSION NO.:NP_003626
PROTEIN GI NO.:4505353
OFFICIAL SYMBOL:NDST2
GENE ID:8509

Background

BACKGROUND:The N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2 (NDST2) protein, also known as N-heparan sulfate sulfotransferase 2, and the highly related protein NDST1 have key roles in the sulfation of heparan sulfate proteoglycans, molecules that are present of cell surfaces and the extracellular matrix (1,2). NDST1 and NDST2 are dispensable for mesodermal differentiation into osteoblasts but necessary for induction of adipocytes and neural cells (3).
REFERENCES: 1) Humphries DE, Lanciotti J, and Karlinsky JB. cDNA cloning, genomic organization and chromosomal localization of human heparan glucosaminyl N-deacetylase/N-sulphotransferase-2. Biochem. J. 1998; 332:303-7.
2) Esko JD and Selleck SB. Order out of chaos. Assembly of ligand binding sites in heparan sulfate. Annu. Rev. Biochem. 2002; 71:435-71.
3) Forsberg M, Holmborn K, Kundu S, et al. Undersulfation of heparan sulfate restricts differentiation potential of mouse embryonic stem cells. J. Biol. Chem. 2012; 287:10853-62.

For Research Use Only