Datasheet

NDFIP1 Antibody
CATALOG NUMBER: 6977

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rat: (84%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:NDFIP1 antibody can be used for detection of NDFIP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1216 - PC-3 Cell Lysate
 2) Cat. No. 10-101 - Human Lung Tissue Slide
PREDICTED MOLECULAR WEIGHT:24 kDa
SPECIFICITY:NDFIP1 antibody is predicted to not cross-react with NDFIP2.
IMMUNOGEN:NDFIP1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human NDFIP1.

The immunogen is located within the first 50 amino acids of NDFIP1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:NDFIP1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:NDFIP1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:NDFIP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:NDFIP1 Antibody: N4WBP5, N4WBP5, PSEC0192, PSEC0223, NEDD4 family-interacting protein 1, Breast cancer-associated protein SGA-1M
ACCESSION NO.:NP_085048
PROTEIN GI NO.:13386480
OFFICIAL SYMBOL:NDFIP1
GENE ID:80762

Background

BACKGROUND:NDFIP1 Antibody: The NEDD4 family-interacting protein 1 (NDFIP1) belongs to a small group of evolutionarily conserved proteins with three transmembrane domains and is an integral Golgi membrane protein. It is a potential target for ubiquitination by the Nedd4 family of proteins. NDFIP1 is strongly expressed in surviving neurons following acute cortical brain injury, and overexpression in cultured cortical neurons increased survival following growth factor starvation, suggesting that NDFIP1 may play a role in neuronal survival. NDFIP1 and the related protein NDFIP2 are thought to interact with and regulate multiple components of the EGF and PTEN/Akt signaling pathways. Recent studies suggest that NDFIP1 may also play a role in Th17 differentiation by limiting the production of proinflammatory cytokines.
REFERENCES: 1) Harvey KF, Shearwin-Whyatt LM, Fotia A, et al. N4WBP5, a potential target for ubiquitination by the Nedd4 family of proteins, is a novel Golgi-associated protein. J. Biol. Chem. 2002; 277:9307-17.
2) Sang Q, Kim MH, Kumar S, et al. Nedd4-WW domain-binding protein 5 (Ndfip1) is associated with neuronal survival after acute cortical brain injury. J. Neurosci. 2006; 26:7234-44.
3) Mund T and Pelham HR. Regulation of PTEN/Akt and MAP kinase signaling pathways by the ubiquitin ligase activators Ndfip1 and Ndfip2. Proc. Natl. Acad. Sci. USA 2010; 107; 11429-34.
4) Ramon HE, Beal AM, Liu Y, et al. The E3 ubiquitin ligase adaptor Ndfip1 regulates Th17 differentiation by limiting the production of proinflammatory cytokines. J. Immunol. 2012; epub.

For Research Use Only