Datasheet

NCLN Antibody
CATALOG NUMBER: 6789

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:NCLN antibody can be used for detection of NCLN by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1401 - Mouse Heart Tissue Lysate
 2) Cat. No. 10-501 - Human Heart Tissue Slide
IMMUNOGEN:NCLN antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NCLN.

The immunogen is located within amino acids 390 - 440 of NCLN.
HOST SPECIES:Rabbit

Properties

PURIFICATION:NCLN Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:NCLN Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:NCLN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:NCLN Antibody: NET59, Nicalin, Nicastrin-like protein
ACCESSION NO.:NP_064555
PROTEIN GI NO.:51873031
OFFICIAL SYMBOL:NCLN
GENE ID:56926

Background

BACKGROUND:NCLN Antibody: Nicalin, along with its binding partner Nomo, is a novel signaling antagonist to Nodals, signaling factors of the TGF-β superfamily that play a key role in vertebrate development. Nicalin is distantly related to Nicastrin, a component of the Alzheimer's disease-associated gamma-secretase, and is thought to have a similar function. Another protein, TMEM147, has also been recently identified as associating with Nicalin and NOMO as part of the gamma-secretase-like complex.
REFERENCES: 1) Haffner C, Frauli M, Topp S, et al. Nicalin and its binding partner Nomo are novel Nodal signaling antagonists. EMBO J. 2004; 23:3041-50.
2) Dettmer U, Kuhn PH, Abou-Ajram C, et al. Transmembrane protein 147 (TMEM147) is a novel component of the Nicalin-NOMO protein complex. J. Biol. Chem. 2010; 285:26174-81.

For Research Use Only