Datasheet

NCBP1 Antibody
CATALOG NUMBER: 7395

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Chicken: (81%)
TESTED APPLICATIONS:ELISA, ICC, WB
APPLICATIONS:NCBP1 antibody can be used for detection of NCBP1 by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 87 kDa

Observed: 87 kDa
SPECIFICITY:NCBP1 antibody is human, mouse and rat reactive. At least two isoforms of NCBP1 are known to exist; this antibody will detect both isoforms of NCBP1.
IMMUNOGEN:NCBP1 antibody was raised against a 17 amino acid peptide near the center of human NCBP1.

The immunogen is located within amino acids 420 - 470 of NCBP1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:NCBP1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:NCBP1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:NCBP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:NCBP1 Antibody: NCBP, Sto1, CBP80, NCBP, Nuclear cap-binding protein subunit 1, 80 kDa nuclear cap-binding protein
ACCESSION NO.:NP_002477
PROTEIN GI NO.:4505343
OFFICIAL SYMBOL:NCBP1
GENE ID:4686

Background

BACKGROUND:NCBP1 Antibody: NCBP1, also known as CBP80, is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. NCBP1 promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay (1,2). Recent evidence has shown that cellular-cap-binding proteins such as NCBP1 associate with influenza virus mRNAs, suggesting that these viral mRNAs may follow the normal cellular pathways for splicing, nuclear export, and translation (3).
REFERENCES: 1) Ohno M, Kataoka N, and Shimura Y. A nuclear cap binding protein from HeLa cells. Nuc. Acid Res. 1990; 18:6989-95.
2) Maquat LE, Hwang J, Sato H, et al. CBP80-promoted mRNP rearrangements during the pioneer round of translation, nonsense-mediated mRNA decay, and thereafter. Cold Spring Harbor Symp. Quant. Biol. 2010; 75:127-34.
3) Bier K, York A, and Fodor E. Cellular cap-binding proteins associate with influenza virus mRNAs. J. Gen. Virol. 92:1627-34.

For Research Use Only