Datasheet

NAT11 Antibody
CATALOG NUMBER: 5549

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:NAT11 antibody can be used for detection of NAT11 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1314 - Human Thymus Tissue Lysate
IMMUNOGEN:NAT11 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human NAT11.

The immunogen is located within the last 50 amino acids of NAT11.
HOST SPECIES:Rabbit

Properties

PURIFICATION:NAT11 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:NAT11 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:NAT11 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:NAT11 Antibody: NAT11, PATT1, NAT11, N-alpha-acetyltransferase 40, N-acetyltransferase 11
ACCESSION NO.:NP_079047
PROTEIN GI NO.:189571650
OFFICIAL SYMBOL:NAA40
GENE ID:79829

Background

BACKGROUND:NAT11 Antibody: N-terminal acetylation is one of the most common protein modifications in eukaryotes, occurring on approximately 57% and 84% on yeast and human proteins respectively. There are several N-terminal acetylating enzyme complexes (NatA - NatE). Unlike the other complexes, NatD is composed of a single protein, NAT11, and has recently been described to acetylate the Serine N-termini of histones H2A and H4 in yeast. The role these modifications play is unknown; yeast that do not express NAT11 grow at normal rates and have no observable phenotypes. The role of the human homolog is likewise unknown.
REFERENCES: 1) Arnesen T, Van Damme P, Polevoda B, et al. Proteomics analyses reveal the evolutionary conservation and divergence of N-terminal acetyltransferases from yeast and humans. Proc. Natl. Acad. Sci. USA2009; 106:8157-62.
2) OK Song, Wang X, Waterborg JH, et al. An Nalpha-acetyl-transferase responsible for acetylation of the N-terminal residues of histones H4 and H2A. J. Biol. Chem.2003; 278:38109-1

For Research Use Only