Nanos3 Antibody Cat. No.: 4653

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Nanos3 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of the human Nanos3.

The immunogen is located within the last 50 amino acids of Nanos3.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: Nanos3 antibody can be used for detection of Nanos3 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: This Nanos3 antibody will not cross-react with either Nanos 1 or Nanos2.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 19 kDa

Observed: 20 kDa

psi-iconProperties
PURIFICATION:Nanos3 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Nanos3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Nanos3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:NANOS3
ALTERNATE NAMES:Nanos3 Antibody: NOS3, NANOS1L, NOS3, Nanos homolog 3, NOS-3
ACCESSION NO.:P60323
PROTEIN GI NO.:41688563
GENE ID:342977
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Nanos3 Antibody: Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In Drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects.
REFERENCES:1) Lehmann R and Nusslein-Volhard C. The maternal gene nanos has a central role in posterior pattern formation of the drosophila embryo. Development 1991; 112:679-91.
2) Tsuda M, Sasaoka Y, Kiso M, et al. Conserved role of nanos proteins in germ cell development. Science 2003; 301:1239-41.
3) Sato K, Hayashi Y, Ninomiya Y, et al. Maternal Nanos represses hid/skl-dependent apoptosis to maintain the germ line in Drosophila embryos. Proc. Natl. Acad. Sci. USA 2007; 104:7455-60.
4) Haraguchi S, Tsuda M, Kitajima S, et al. Nanos1: a mouse nanos gene expressed in the central nervous system is dispensable for normal development. Mech. Dev. 2003; 120:721-31.

ANTIBODIES FOR RESEARCH USE ONLY.

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