Datasheet

Nanos1 Antibody
CATALOG NUMBER: 4685

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:Nanos1 antibody can be used for detection of Nanos1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
IMMUNOGEN:Nanos1 antibody was raised against a 14 amino acid synthetic peptide from near the center of human Nanos1.

The immunogen is located within amino acids 160 - 210 of Nanos1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Nanos1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Nanos1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Nanos1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Nanos1 Antibody: NOS1, SPGF12, NOS1, Nanos homolog 1, NOS-1
ACCESSION NO.:Q8WY41
PROTEIN GI NO.:41688589
OFFICIAL SYMBOL:NANOS1
GENE ID:340719

Background

BACKGROUND:Nanos1 Antibody: Nanos1 is one of three known mammalian homologs to the Drosophila gene nanos. Nanos1 is an RNA-binding protein containing a zinc-finger motif and is expressed in the developing nervous system and continues in the adult brain. Interestingly, unlike mice deficient in either nanos2 or nanos3, mice lacking the nanos1 gene develop normally with no sign of abnormalities. Recently it has been found that expression of nanos1 mRNA is down-regulated by E-cadherin in a human breast cancer cell line and the amino-terminal domain on Nanos1 interacts with the E-cadherin-binding protein p120ctn. Furthermore, overexpression of Nanos1 in human colorectal DLD1 cancer cells functionally abolished cell-cell adhesion, allowing the cancer cells to develop strong migratory and invasive properties. These results suggest that targeting Nanos1 might prove an effective strategy in the treatment of E-cadherin-negative tumors.
REFERENCES: 1) Jaruleska J, Kotecki M, Kusz K, et al. Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells. Dev. Genes Evol.2003; 213:120-6.
2) Tsuda M, Sasaoka Y, Kiso M, et al. Conserved role of nanos proteins in germ cell development. Science2003; 301:1239-41.
3) Haraguchi S, Tsuda M, Kitajima S, et al. Nanos1: a mouse nanos gene expressed in the central nervous system is dispensable for normal development. Mech. Dev.2003; 120:721-31.
4) Strumane K, Bonnomet A, Stove A, et al. E-cadherin regulates human Nanos1, which interacts with p120ctn and induces tumor cell migration and invasion. Cancer Res.2006; 66:10007-15.

For Research Use Only