Datasheet

NALP5 Antibody
CATALOG NUMBER: 5959

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:NALP5 antibody can be used for detection of NALP5 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1403 - Mouse Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
IMMUNOGEN:NALP5 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human NALP5.

The immunogen is located within the last 50 amino acids of NALP5.
HOST SPECIES:Rabbit

Properties

PURIFICATION:NALP5 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:NALP5 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:NALP5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:NALP5 Antibody: MATER, NALP5, PAN11, PYPAF8, CLR19.8, MATER, NACHT, LRR and PYD domains-containing protein 5, Mater protein homolog
ACCESSION NO.:NP_703148
PROTEIN GI NO.:158321897
OFFICIAL SYMBOL:NLRP5
GENE ID:126206

Background

BACKGROUND:NALP5 Antibody: NALP proteins include the apoptosis regulator APAF1 (apoptotic protease activating factor 1) and mammalian NOD-LRR proteins and are thought to be involved in inflammation and reproduction. NALP5, also known as MATER, is a maternal gene required for early embryonic development in mice. Increased NALP5 expression was observed in two neuronal injury models, and transient expression of recombinant NALP5 in neurons induced caspase-3 activation and apoptosis, suggesting that NALP5 also plays a role in caspase activation and apoptosis in injured neurons, and may thus represent a novel target for therapeutic treatment in neurodegenerative disorders.
REFERENCES: 1) Tschopp J, Martinon F, and Burns K. NALPs: a novel protein family involved in inflammation. Nat. Rev. Mol. Cell Biol.2003; 4:95-104.
2) Tian X, Pascal G, and Monget P. Evolution and functional divergence of NLRP genes in mammalian reproductive system. BMC Evol. Biol.2009; 9:202.
3) Tong ZB, Gold L, Pfeiffer KE, et al. Mater, a maternal effect gene required for early embryonic development in mice. Nat. Genet.2000; 26:267-8.
4) Frederick Lo C, Ning X, Gonzales C, et al. Induced expression of death domain genes NALP1 and NALP5 following neuronal injury. Biochem. Biophys. Res. Commun.2008; 366:664-9.

For Research Use Only