Datasheet

NALP3 Antibody
CATALOG NUMBER: 5447

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (88%)
TESTED APPLICATIONS:IF
APPLICATIONS:NALP3 antibody can be used for detection of NALP3 by Western blot at 1 μg/mL. Antibody can also be used for Immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate
 2) Cat. No. 17-004 - K-562 Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 101, 114 kDa

Observed: 110 kDa
IMMUNOGEN:NALP3 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human NALP3.

The immunogen is located within amino acids 120 - 170 of NALP3.
HOST SPECIES:Rabbit

Properties

PURIFICATION:NALP3 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:NALP3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:NALP3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:NALP3 Antibody: AII, AVP, FCU, MWS, FCAS, CIAS1, NALP3, C1orf7, CLR1.1, PYPAF1, AGTAVPRL, NACHT, LRR and PYD domains-containing protein 3, Cold autoinflammatory syndrome 1 protein
ACCESSION NO.:NP_899632
PROTEIN GI NO.:34878690
OFFICIAL SYMBOL:NLRP3
GENE ID:114548

Background

BACKGROUND:NALP3 Antibody: NALP3, a member of Nod-like receptors, has a crucial role in inflammation and immunity and may be a proximal sensor of cellular stress and danger signals. NALP3 forms a caspase-1 activating molecular complex termed the inflammasome. The inflammasome allows activation of IL-1β, the key player of the inflammation and fever. NALP3 gene encodes a pyrin like protein which contains a pyrin domain, a nucleotide binding site (NBS) domain, and a leucine rich repeat (LRR) motif. NALP3 protein interacts with apoptosis associated speck like protein containing a CARD and may function as an inducer of apoptosis and an activator of NF-κB signaling. Defects in NALP3 have been associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID).
REFERENCES: 1) Chamaillard M, Girardin SE, Viala J, et al. Nods, Nalps and Naip: intracellular regulators of bacterial-induced inflammation. Cell. Microbiol.2003; 5:581-92.
2) O'Connor W Jr., Hartin JA, Zhu X, et al. Cutting edge: CIAS1/cryopyrin/PYPAF1/NALP3/CATERPILLER 1.1 is an inducible inflammatory mediator with NF-kappa B suppressive properties. J. Immunol.2003; 171:6329-33.
3) Kanneganti TD, Ozoren N, Body-Malapal M, et al. Bacterial RNA and small antiviral compounds activate caspase-1 through cryopyrin/Nalp. Nature2006; 440:233-6.
4) Agostini L, Martinon F, Burns K, et al. NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder. Immunity 2004; 20:319-25.

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