Datasheet

NALP2 Antibody
CATALOG NUMBER: 3027

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:NALP2 antibody can be used for detection of NALP2 by Western blot at 1 to 2 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1216 - PC-3 Cell Lysate
IMMUNOGEN:NALP2 antibody was raised against a peptide corresponding to 12 amino acids near the carboxy terminus of human NALP2.

The immunogen is located within the last 50 amino acids of NALP2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:NALP2 Antibody is immunoaffinity chromatography purified IgG.
PHYSICAL STATE:Liquid
BUFFER:NALP2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:NALP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:NALP2 Antibody: NBS1, PAN1, NALP2, PYPAF2, CLR19.9, NBS1, NACHT, LRR and PYD domains-containing protein 2, Nucleotide-binding site protein 1
ACCESSION NO.:NP_060322
PROTEIN GI NO.:8923473
OFFICIAL SYMBOL:NLRP2
GENE ID:55655

Background

BACKGROUND:NALP2 Antibody: NALP2 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli. Unlike the prototypical NALP protein NALP1, NALP2 only contains a NACHT domain, leucine rich repeat (LRR), and pyrin-containing domain (PYD). This protein interacts with the adapter protein ASC in addition to CARD8 and caspase-1 to form an inflammasome with high proIL-1β-processing activity and is thought to function as a modulator of NF-κB and procaspase-1 activation in macrophages. It has also been suggested that NALP2, in addition to other NALP family members, can act as innate sensors of pathogens in a manner similar to the toll-like receptors (TLRs). At least two alternatively spliced transcript variants encoding distinct isoforms have been found for NALP2.
REFERENCES: 1) Tschopp J, Martinon F, and Burns K. NALPs: a novel protein family involved in inflammation. Nat. Rev. Mol. Cell Biol. 2003; 4:95-104.
2) Bruey JM, Bruey-Sedano N, Newman R, et al. PAN1/NALP2/PYPAF2, an inducible inflammatory mediator that regulates NF-κB and caspase-1 activation in macrophages. J. Biol. Chem. 2004; 279:51897-907.
3) Agostini L, Martinon F, Burns K, et al. NALP3 forms an IL-1β-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder. Immunity 2004; 20:319-25.
4) Martinon F and Tschopp J. NLRs join TLRs as innate sensors of pathogens. TRENDS Imm. 2005; 26:447-54.

For Research Use Only