Datasheet

NAK Antibody
CATALOG NUMBER: 2351

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, ICC, WB
APPLICATIONS:NAK antibody can be used for detection of NAK/TBK1 by Western blot. An 84 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.

Antibody validated: Immunocytochemistry in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1206 - MOLT4 Cell Lysate
 2) Cat. No. 17-006 - MOLT-4 Cell Slide
PREDICTED MOLECULAR WEIGHT:84 kDa
SPECIFICITY:No cross response to IKKa, IKKb, IKKy, or IKKe.
IMMUNOGEN:NAK antibody was raised against a synthetic peptide corresponding to 17 amino acids form near the carboxy terminus of human NAK/TBK1.

The immunogen is located within the last 50 amino acids of NAK.
HOST SPECIES:Rabbit

Properties

PURIFICATION:NAK Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:NAK Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:NAK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:NAK Antibody: NAK, T2K, NAK, NF-kappa-B-activating kinase
ACCESSION NO.:NP_037386
PROTEIN GI NO.:7019547
OFFICIAL SYMBOL:TBK1
GENE ID:29110

Background

BACKGROUND:NAK Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. Phosphorylation of I-kappaB by IκB kinase (IKK) complex leads to degradation of I-kappaB and activation of NF-κB. The IKK complex contains IKKα, IKKβ, and IKKγ. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-κB-activating kinase), and T2K. NAK/TBK1 activates IKKβ through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-κB activation in response to growth factors. NAK/TBK1 functions upstream of NIK and the IKK complex. NAK/TBK1 is also critical in protecting embryonic liver from apoptosis.
REFERENCES: 1) Pomerantz JL, Baltimore D. NF-κB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase. EMBO J 1999;18(23):6694-704
2) Tojima Y, Fujimoto A, Delhase M, Chen Y, Hatakeyama S, Nakayama K, Kaneko Y, Nimura Y, Motoyama N, Ikeda K, Karin M, Nakanishi M. NAK is an IκB kinase-activating kinase. Nature 2000;404(6779):778-82
3) Bonnard M, Mirtsos C, Suzuki S, Graham K, Huang J, Ng M, Itie A, Wakeham A, Shahinian A, Henzel WJ, Elia AJ, Shillinglaw W, Mak TW, Cao Z, Yeh WC. Deficiency of T2K leads to apoptotic liver degeneration and impaired NF-κB-dependent gene transcription. EMBO J. 2000;19(18):4976-85.

For Research Use Only