Datasheet

NAIP Antibody
CATALOG NUMBER: 3315

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (92%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:NAIP antibody can be used for the detection of NAIP by Western blot at 0.5 to 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1315 - Human Tonsil Tissue Lysate
 2) Cat. No. 1203 - A549 Cell Lysate
 3) Cat. No. 17-003 - A549 Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 137, 154 kDa

Observed: 155 kDa
SPECIFICITY:Minor, lower molecular weight bands may represent alternately spliced forms.
IMMUNOGEN:NAIP antibody was raised against a synthetic peptide corresponding to 13 amino acids at the C-terminus of human NAIP.

The immunogen is located within amino acids 1200 - 1250 of NAIP.
HOST SPECIES:Rabbit

Properties

PURIFICATION:NAIP Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:NAIP Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:NAIP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:NAIP Antibody: BIRC1, NLRB1, psiNAIP, BIRC1, Baculoviral IAP repeat-containing protein 1, Neuronal apoptosis inhibitory protein
ACCESSION NO.:AAC52047
PROTEIN GI NO.:1737213
OFFICIAL SYMBOL:NAIP
GENE ID:4671

Background

BACKGROUND:NAIP Antibody: Neuronal apoptosis inhibitor protein (NAIP) was the first human inhibitor of apoptosis protein (IAP) identified and was discovered by its association with the neurodegenerative disorder spinal muscular atrophy. Members of the IAP family contain one to three copies of an approximately 70 amino acid motif termed baculovirus IAP repeat (BIR); these BIRs promote protein-protein interactions with various caspases such as caspase-3, -7, and -9 as well as members of the TRAF family of signal molecules. Unlike other IAPs however, NAIP requires ATP to bind caspase-9 and is not inhibited by the IAP-inhibiting molecule Smac/DIABLO, suggesting that NAIP is unique among the IAPs in its regulation of its activity. Finally, although only one human NAIP protein has been identified, other shorter NAIP mRNA transcripts have been reported.
REFERENCES: 1) Roy N, Mahadevan MS, McLean M, et al. The gene for neuronal apoptosis inhibitory protein is partially deleted in individuals with spinal muscular atrophy. Cell 1995; 80:167-78.
2) Liston P, Fong WG, Korneluk RG. The inhibitors of apoptosis: there is more to life than Bcl2. Oncogene 2003; 22:8568-80.
3) Verhagen AM, Coulson EJ, and Vaux DL. Inhibitor of apoptosis proteins and their relatives: IAPs and other BIRPs. Genome Biol. 2001; 2:reviews3009.1-reviews3009.10.
4) Davoodi J, Lin L, Kelly J, et al. Neuronal apoptosis-inhibitory protein does not interact with Smac and requires ATP to bind caspase-9. J. Biol. Chem. 2004; 279:40622-8.

For Research Use Only