N4BP1 Antibody Cat. No.: 6175

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: N4BP1 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human N4BP1.

The immunogen is located within the first 50 amino acids of N4BP1.
TESTED APPLICATIONS: ELISA, ICC, IF, WB
APPLICATIONS: N4BP1 antibody can be used for detection of N4BP1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: At least three isoforms of N4BP1 are known to exist; this antibody will only detect the longest isoform.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide

psi-iconProperties
PURIFICATION:N4BP1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:N4BP1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:N4BP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:N4BP1
ALTERNATE NAMES:N4BP1 Antibody: KIAA0615, NEDD4-binding protein 1, N4BP1
ACCESSION NO.:NP_694574
PROTEIN GI NO.:48928019
GENE ID:9683
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:N4BP1 Antibody: Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins and may have a fundamental role to play in embryonic processes. N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. N4BP1 also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.
REFERENCES:1) Murillas R, Simms KS, Hatakeyama S, et al. Identification of developmentally expressed proteins that functionally interact with Nedd4 ubiquitin ligase. J. Biol. Chem. 2002; 277:2897-907.
2) Sharma P, Murillas R, Zhang H, et al. N4BP1 is a newly identified nucleolar protein that undergoes SUMO-regulated polyubiquitylation and proteasomal turnover at promyelocytic leukemia nuclear bodies. J. Cell Sci. 2010; 123:1227-34.
3) Oberst A, Malatesta M, Aqeilan RI, et al. The Nedd4-binding partner 1 (N4BP1) protein is an inhibitor of the E3 ligase Itch. Proc. Natl. Acad. Sci. USA 2007; 104:11280-5.

ANTIBODIES FOR RESEARCH USE ONLY.

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