N-RAS Antibody Cat. No.: 7381

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Pig: (100%), Guinea pig: (93%), Chicken: (80%)
IMMUNOGEN: N-RAS antibody was raised against a 15 amino acid peptide near the carboxy terminus of human N-RAS.

The immunogen is located within the last 50 amino acids of N-RAS.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: N-RAS antibody can be used for detection of N-RAS by Western blot at 1 and 2 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
SPECIFICITY: N-RAS antibody is predicted to not cross-react with H-RAS
POSITIVE CONTROL:1) Cat. No. 1202 – A431 Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 22 kDa

Observed: 22 kDa

psi-iconProperties
PURIFICATION:N-RAS Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:N-RAS Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:N-RAS antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:NRAS
ALTERNATE NAMES:N-RAS Antibody: NS6, ALPS4, N-ras, NRAS1, HRAS1, GTPase NRas, Transforming protein N-Ras
ACCESSION NO.:NP_002515
PROTEIN GI NO.:4505451
GENE ID:4893
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:N-RAS Antibody: Activating mutations and overexpression of classical Ras subfamily members (N-RAS, H-RAS, and K-RAS) have been widely investigated as key events in the development of human cancers. The N-RAS protein shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. N-RAS, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.
REFERENCES:1) Ehrhardt A, Ehrhardt GR, Guo X, et al. Ras and relatives--job sharing and networking keep an old family together. Exp. Hematol. 2002; 30:1089-106.
2) Swarthout JT, Lobo S, Farh L, et al. DHHC9 and GCP9 constitute a human protein fatty acyltransferase with specificity for H- and N-Ras. J. Biol. Chem. 2005; 280:31141-8.
3) Malaney S and Daly RJ. The ras signaling pathway in mammary tumorigenesis and metastasis. J. Mammary Gland Biol. Neoplasia 2001; 6:101-13.
4) Rodenhuis S. ras and human tumors. Semin Cancer Biol. 1992; 3:241-7

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