MYOZAP Antibody Cat. No.: 6223

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: MYOZAP antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human MYOZAP.

The immunogen is located within amino acids 400 - 450 of MYOZAP.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: MYOZAP antibody can be used for detection of MYOZAP by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: Multiple isoforms of MYOZAP are known to exist.
POSITIVE CONTROL:1) Cat. No. 1465 - Rat Kidney Tissue Lysate
 2) Cat. No. 10-101 - Human Lung Tissue Slide

psi-iconProperties
PURIFICATION:MYOZAP Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:MYOZAP Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MYOZAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:MYZAP
ALTERNATE NAMES:MYOZAP Antibody: gcom, Gcom2, MYZAP, GRINL1A, MYZAP-POLR2M, MYOZAP, Myocardial zonula adherens protein, GRINL1A upstream protein, Gup
ACCESSION NO.:NP_001018110
PROTEIN GI NO.:70166414
GENE ID:100820829
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:MYOZAP Antibody: MYOZAP, also known as GRINL1A, is a 54 kDa highly conserved cardiac protein. It is strongly expressed in the heart and lung and is a novel component of intercalated disc. MYOZAP interacts with myosin phosphatase-RhoA interacting protein (MRIP) and acts as an activator of Rho-dependent SRF signaling. Knockdown study in zebrafish results in cardiomyopathy with severe dysfunction. The MYOZAP gene is part of a complex transcript unit that includes the gene for glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (GRINL1A). Transcription of this gene occurs at an upstream promoter, with two different groups of alternatively spliced variants: Gup for GRINL1A upstream transcripts and Gcom for GRINL1A combined transcripts.
REFERENCES:1) Van Tintelen JP, Hofstra RM, Wiesfeld AC, et al. Molecular genetics of arrhythmogenic right ventricular cardiomyopathy: emerging horizon? Curr. Opin. Cardiol. 2007; 22:185-92.
2) Seeger TS, Frank D, Rohr C, et al. Myozap, a novel intercalated disc protein, activates serum response factor-dependent signaling and is required to maintain cardiac function in vivo. Circ. Res. 2010; 106:880-90.
3) Roginski RS, Mohan Raj BK, Birditt B, et al. The human GRINL1A gene defines a complex transcription unit, an unusual form of gene organization in eukaryotes. Genomics 2004; 84:265-76.

ANTIBODIES FOR RESEARCH USE ONLY.

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