| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Horse: (95%), Rabbit: (95%), Pig: (89%), Bovine: (89%), Chicken: (89%) |
| IMMUNOGEN: | MYH8 antibody was raised against a 19 amino acid peptide near the amino terminus of human MYH8. The immunogen is located within amino acids 60 - 110 of MYH8. |
| TESTED APPLICATIONS: | ELISA, IF, IHC-P, WB |
| APPLICATIONS: | MYH8 antibody can be used for detection of MYH8 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
| SPECIFICITY: | MYH8 antibody is human, mouse and rat reactive. MYH8 antibody is predicted to not cross-react with other members of the myosin heavy chain family. |
| POSITIVE CONTROL: | 1) Cat. No. 1201 - HeLa Cell Lysate |
| PREDICTED MOLECULAR WEIGHT: | Predicted: 213 kDa Observed: 220 kDa |
Specifications| PURIFICATION: | MYH8 antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | MYH8 antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | MYH8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| OFFICIAL SYMBOL: | MYH8 |
| ALTERNATE NAMES: | Myosin heavy chain 8, Myosin-8, Myosin heavy chain skeletal muscle perinatal, MyHC-perinatal, MyHC-peri, MyHC-pn, gtMHC-F |
| ACCESSION NO.: | NP_002463 |
| PROTEIN GI NO.: | 153945790 |
| GENE ID: | 4626 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells (1). MYH8 (myosin, heavy chain 8, skeletal muscle, perinatal) is a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction (2,3). This gene is predominantly expressed in fetal skeletal muscle. MYH8 is regulated by phosphorylation via myosin light chain kinase (MLCK) and by intracellular Ca2+ concentrations (3). A mutation in this gene results in trismus-pseudocamptodactyly syndrome (4). |
| REFERENCES: | 1) Yu H, Waddell JN, Kuang S, et al. Park7 expression influences myotube size and myosin expression in muscle. PLoS One 2014; 9:e92030. | 2) Feghali R and Leinwand LA. Molecular genetic characterization of a developmentally regulated human perinatal myosin heavy chain. J. Cell Biol. 1989; 108:1791-7. | 3) Jullian EH, Kelly AM, Pompidou AJ, et al. Characterization of a human perinatal myosin heavy-chain transcript. Eur. J. Biochem. 1995; 230:1001-6. | 4) Minzer-Conzetti K, Wu E, Vargervik K, et al. Phenotypic variation in trismus-pseudocamptodactyly syndrome caused by a recurrent MYH8 mutation. Clin. Dysmorphol. 2008; 17:1-4. |
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