Datasheet

MYBPC2 Antibody
CATALOG NUMBER: 5651

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Chicken: (91%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:MYBPC2 antibody can be used for detection of MYBPC2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1407 - Mouse Skeletal Muscle Tissue Lysate
IMMUNOGEN:MYBPC2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human MYBPC2.

The immunogen is located within amino acids 1070 - 1120 of MYBPC2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:MYBPC2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MYBPC2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MYBPC2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MYBPC2 Antibody: MYBPC, MYBPCF, Myosin-binding protein C, fast-type, C-protein, skeletal muscle fast isoform, Fast MyBP-C
ACCESSION NO.:EAW71866
PROTEIN GI NO.:119592272
OFFICIAL SYMBOL:MYBPC2
GENE ID:4606

Background

BACKGROUND:MYBPC2 Antibody: Myosin binding protein C (MYBPC) is a component of the thick filament of striated muscle, with the fast-type isoform designated MYBPC2. Both the fast-type and slow-type MYBPC protein contains seven immunoglobulin C2 motifs and three fibronectin type-III repeats. MYBPC2 is typically required for strong contractions and functions under anaerobic conditions. It is more similar to the cardiac isoform (MYBPC3) than to the slow-type isoform (MYBPC1) in terms of pCa50-indexed force development, length-independent cooperativity and length dependent activation. It has been suggested that in cardiac and fast muscle MYBPC2 contributes to an internal load, possibly by binding to actin via its N-terminal region.
REFERENCES: 1) Weber FE, Vaughan KT, Reiach FC, et al. Complete sequence of human fast-type and slow-type muscle myosin-binding-protein C (MyBP-C). Differential expression, conserved domain structure and chromosome assignment. Eur. J. Biochem.1993; 216:661-9.
2) Konhilas JP, Irving TC and De Tombe PP. Length-dependent activation in three striated muscle types of the rat. J. Physiol.2002; 544:225-36.
3) Flashman E, Korkie L, Watkins H, et al. Support for a trimeric collar of myosin binding protein C in cardiac and fast skeletal muscle, but not in slow skeletal muscle. FEBS Lett.2008; 582:434-8.

For Research Use Only