Munc-18 (phospho Ser515) Antibody Cat. No.: 50-230

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Bovine, Chicken, Dog, Human, Mouse, Rat, Xenopus
IMMUNOGEN: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser515 of Munc-18.
TESTED APPLICATIONS: IHC, WB
APPLICATIONS: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primates and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
PREDICTED MOLECULAR WEIGHT: 65

psi-iconProperties
PURIFICATION:Affinity Purified
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
STORAGE CONDITIONS:Munc-18 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

psi-iconAdditional Info
OFFICIAL SYMBOL:Stxbp2
ACCESSION NO.:Q62753
PROTEIN GI NO.:2501516
GENE ID:81804
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Munc-18 ( mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc-18 is thought to be regulated by PKC phosphorylation of Ser515 on the Munc-18 protein (Sassa et al., 1996).
REFERENCES:1) Ciufo LF, Barclay JW, Burgoyne RD, Morgan A (2005) Munc18-1 regulates early and late stages of exocytosis via syntaxin-independent protein interactions. Mol Biol Cell 16:470-482.
2) Liu J, Ernst SA, Gladycheva SE, Lee YY, Lentz SI, Ho CS, Li Q, Stuenkel EL (2004) Fluorescence resonance energy transfer reports properties of syntaxin1a interaction with Munc18-1 in vivo. J Biol Chem 279:55924-55936.
3) Sassa T, Ogawa H, Kimoto M, Hosono R (1996) The synaptic protein UNC-18 is phosphorylated by protein kinase C. Neurochem Int 29:543-552.

ANTIBODIES FOR RESEARCH USE ONLY.

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