Datasheet

MUC1 Antibody
CATALOG NUMBER: 7451

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (83%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:MUC1 antibody can be used for detection of MUC1 by Western blot at 1 - 2 μg/ml.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 53 (+ multiple others) kDa

Observed:42, 120 kDa
SPECIFICITY:MUC1 antibody is human and mouse reactive. Multiple isoforms of MUC1 are known to exist. MUC1 often migrates at a higher molecular weight in SDS-PAGE due to high levels of post-translational modification.
IMMUNOGEN:MUC1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human MUC1.

The immunogen is located within the last 50 amino acids of MUC1 .
HOST SPECIES:Rabbit

Properties

PURIFICATION:MUC1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MUC1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MUC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MUC1 Antibody : EMA, MCD, PEM, PUM, KL-6, MAM6, MCKD, PEMT, CD227, H23AG, MCKD1, MUC-1, ADMCKD, ADMCKD1, CA 15-3, MUC-1/X, MUC1/ZD, MUC-1/SEC, Mucin-1, Breast carcinoma-associated antigen DF3
ACCESSION NO.:NP_001191215
PROTEIN GI NO.:324120958
OFFICIAL SYMBOL:MUC1
GENE ID:4582

Background

BACKGROUND:Mucin 1 (MUC1) is membrane-bound protein member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces and also play a role in intracellular signaling (1). MUC1 is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. MUC1 is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas (1).
REFERENCES: 1) Singh R and Bandyopadhyay D. MUC1: a target molecule for cancer therapy. Cancer Biol. Ther. 2007; 6:481-6.

For Research Use Only