Datasheet

MTERFD1 Antibody
CATALOG NUMBER: 7281

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rat: (88%), Chicken: (88%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:MTERFD1 Antibody can be used for detection of MTERFD1 by Western blot starting at 1 μg/mL. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
 2) Cat. No. 17-201 - 3T3/BALB Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 46 kDa

Observed: 42 kDa
SPECIFICITY:At least three isoforms of MTERFD1 are known to exist. MTERFD1 antibody is predicted to not cross-react with other MTERFD protein family members.
IMMUNOGEN:MTERFD1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human MTERFD1 .

The immunogen is located within the last 50 amino acids of MTERFD1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:MTERFD1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MTERFD1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MTERFD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MTERFD1 Antibody: CGI-12, mTERF3, mTERF domain-containing protein 1, mitochondrial, Mitochondrial transcription termination factor 3
ACCESSION NO.:NP_057026
PROTEIN GI NO.:34147676
OFFICIAL SYMBOL:MTERFD1
GENE ID:51001

Background

BACKGROUND:MTERFD1 Antibody: Members of the mTERF (mitochondrial transcription termination factor) family, are mitochondrial proteins that are believed to be transcription termination factors. MTERFD1 is composed of three leucine zippers that form a three-stranded coiled-coil that binds to DNA. It binds promoter DNA and acts as a mitochondrial transcription regulator. It has been suggested that only the phosphorylated form of MTERFD1 has transcription termination activity. A closely related mTERF family member, MTERFD3, is believed to be involved in cell cycle regulation and cell growth by modulating mitochondrial transcription.
REFERENCES: 1) Fernandez-Silva P, et al. The human mitochondrial transcription termination factor (mTERF) is a multizipper protein but binds to DNA as a monomer, with evidence pointing to intramolecular leucine zipper interactions. EMBO J. 1997; 16:1066-79.
2) Hyvarinen AK, Pohjoismaki JL, Holt IJ, et al. Overexpression of MTERFD1 or MTERFD3 impairs the completion of mitochondrial DNA replication. Mol. Biol. Rep. 2011; 38:1321-8.
3) Prieto-Martin A, Montoya J, Martinez-Azorin F, et al. Phosphorylation of rat mitochondrial transcription termination factor (mTERF) is required for transcription termination but not for binding to DNA. Nucleic Acids Res. 2004; 32:2059-68.
4) Park CB, Asin-Cayuela J, Camara Y, et al. MTERF3 is a negative regulator of mammalian mtDNA transcription. Cell 2007; 130:273-85.

For Research Use Only