MSI2 Antibody Cat. No.: 6309

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Rat
IMMUNOGEN: MSI2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human MSI2.

The immunogen is located within amino acids 30 - 80 of MSI2.
TESTED APPLICATIONS: ICC, IF, IHC-P
APPLICATIONS: MSI2 antibody can be used for detection of MSI2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPECIFICITY: MSI2 antibody is predicted to not cross-react with MSI1. Multiple isoforms of MSI2 are known to exist.
POSITIVE CONTROL:1) Cat. No. 1287 - EL4 Cell Lysate
 2) Cat. No. 17-207 - EL4 Cell Slide

psi-iconProperties
PURIFICATION:MSI2 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:MSI2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MSI2 antibody can be stored at 4 ˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:MSI2
ALTERNATE NAMES:MSI2 Antibody: MSI2H, RNA-binding protein Musashi homolog 2, Musashi-2
ACCESSION NO.:EAW94506
PROTEIN GI NO.:20373175
GENE ID:124540
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:MSI2 Antibody: Musashi2 (MSI2) is an RNA-binding protein that is highly expressed in precursor cells in the ventricular and subventricular zones of the developing mammalian CNS. Like the related MSI1, MSI2 has been suggested to be involved stem cell production and maintenance. MSI2 is the predominant MSI protein in hematopoietic stem cells, and its knockdown leads to reduced engraftment and depletion in vivo. Expression levels of MSI2 are elevated in myeloid leukemia cells lines, and MSI2 appears to cooperate with BCR-ABL1 to induce an aggressive leukemia; the level of MSI2 directly correlates with decreased survival in patients. MSI2 negatively regulates the asymmetric cell fate determinant NUMB, suggesting that this signaling pathway may provide future targets for future therapies.
REFERENCES:1) Sakakibara S, Nakamura Y, Stoh H, et al. RNA-binding protein Musashi2: developmentally regulated expression in neuronal precursor cells and subpopulations of neurons in mammalian CNS. J. Neurosci. 2001; 21:8091-107
2) Kharas MG, Lengner CJ, Al-Shahrour F, et al. Musashi-2 regulates normal hematopoiesis and promotes aggressive myeloid leukemia. Nat. Med. 2010; 16:903-8
3) Ito T, Kwon HY, Zimdahl B, et al. Regulation of myeloid leukaemia by the cell-fate determinant Musashi. Nature 2010; 466:765-8.
4) Griner LN and Reuther GW. Aggressive myeloid leukemia formation is directed by the Musashi2/Numb pathway. Cancer Biol. Ther. 2010; 10:979-82

ANTIBODIES FOR RESEARCH USE ONLY.

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