Datasheet

MRE11 Antibody
CATALOG NUMBER: 5547

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Chicken: (93%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:MRE11 antibody can be used for detection of MRE11 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1203 - A549 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 78 kDa

Observed: 73 kDa
IMMUNOGEN:MRE11 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus human MRE11.

The immunogen is located within amino acids 40 - 90 of MRE11.
HOST SPECIES:Rabbit

Properties

PURIFICATION:MRE11 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MRE11 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MRE11 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MRE11 Antibody: ATLD, HNGS1, MRE11, MRE11B, Double-strand break repair protein MRE11A, Meiotic recombination 11 homolog 1, MRE11 homolog 1
ACCESSION NO.:EAW66932
PROTEIN GI NO.:119587336
OFFICIAL SYMBOL:MRE11A
GENE ID:4361

Background

BACKGROUND:MRE11 Antibody: MRE11 is involved in the repair of DNA double strand breaks as part of a complex that includes the Rad50 and NBS1 protein and is thought to act in the same pathway as the A-T mutated (ATM) protein. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for non-homologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. Mutations in this protein result in a novel ataxia telangiectasia-like disorder (ATLD). Unlike the ATM protein, MRE11 is necessary proper mammalian development.
REFERENCES: 1) Stewart GS, Maser RS, Stankovic T, et al. The DNA double-strand break repair gene hMRE11 is mutated in individuals with an ataxia telangiectasia-like disorder. Cell1999; 99:577-87.
2) Buis J, Wu Y, Deng Y, et al. Mre11 nuclease activity has essential roles in DNA repair and genomic stability distinct from ATM activation. Cell2008; 135:85-96.

For Research Use Only