Datasheet

MPYS Antibody
CATALOG NUMBER: 5269

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (82%), Pig: (82%), Chicken: (71%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:MPYS antibody can be used for detection of MPYS by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate
 2) Cat. No. 1205 - Jurkat Cell Lysate
 3) Cat. No. 1288 - A20 Cell Lysate
 4) Cat. No. 17-208 - A-20 Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 31, 42 kDa

Observed: 32 kDa
IMMUNOGEN:MPYS antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human MPYS.

The immunogen is located within amino acids 260 - 310 of MPYS.
HOST SPECIES:Rabbit

Properties

PURIFICATION:MPYS Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MPYS Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MPYS antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MPYS Antibody: ERIS, MITA, MPYS, NET23, STING, ERIS, Stimulator of interferon genes protein, Endoplasmic reticulum interferon stimulator, hSTING
ACCESSION NO.:NP_938023
PROTEIN GI NO.:38093659
OFFICIAL SYMBOL:TMEM173
GENE ID:340061

Background

BACKGROUND:MPYS Antibody: MPYS is a recently identified plasma membrane tetraspanner that is associated with major histocompatibility complex class II (MHC-II) and mediates its transduction of apoptotic signals. It has also been found to be associated with VISA, a mitochondrial protein that acts as an adaptor in virus-triggered signaling. MPYS also interacts with IRF3 and recruits the kinase TBK1 to the VISA-associated complex, acting as a critical mediator of virus-triggered IRF3 activation and interferon (IFN) expression. It is thought that the binding of nucleic acid to the innate immune protein RIG-I causes complex formation between RIG-I, VISA, and MPYS. This complex then recruits TBK1 to phosphorylate IRF3 which then directly activates IFN transcription. At least three isoforms of MPYS are known to exist.
REFERENCES: 1) Jin L, Waterman PM, Jonscher KR, et al. MPYS, a novel membrane tetraspanner, is associated with major histocompatibility complex class II and mediates transduction of apoptotic signals. Mol. Cell. Biol.2008; 28:5014-26.
2) Zhong B, Yang Y, Li S, et al. The adaptor protein MITA links virus-sensing receptors to IRF3 transcription factor activation. Immunity2008; 29:538-50.

For Research Use Only