MPYS Antibody Cat. No.: 5269

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat (82%), Bovine: (82%), Pig: (82%), Chicken: (71%)
IMMUNOGEN: Anti-MPYS antibody (5269) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human MPYS.

The immunogen is located within amino acids 260-310 of MPYS.
TESTED APPLICATIONS: ELISA, ICC, IF, WB
APPLICATIONS: WB: 1-2 μg/mL; ICC: 5 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry and Immunofluorescence in mouse samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate
 2) Cat. No. 1205 - Jurkat Cell Lysate
 3) Cat. No. 1288 - A20 Cell Lysate
 4) Cat. No. 17-208 - A-20 Cell Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 42kD

Observed: 42 kD
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 1) shows similar MPYS expression profile in human cell lines detected by two independent anti-MPYS antibodies that recognize different epitopes, 5269 against near C-terminus domain and 64-236 against C-terminus domain.  MPYS proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

ISOFORMS: Human MPYS has one isoform (379aa, 42kD). Mouse MPYS has 3 isoforms, including isoform 1 (379aa, 42kD), isoform 2 (427aa, 48kD) and isoform 3 (337aa, 38kD). Rat MPYS also has one isoform (379aa, 43kD). 5269 can detect human, mouse and rat.

psi-iconProperties
PURIFICATION:MPYS Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:MPYS Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MPYS antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:TMEM173
ALTERNATE NAMES:MPYS Antibody: ERIS, MITA, MPYS, NET23, STING, ERIS, Stimulator of interferon genes protein, Endoplasmic reticulum interferon stimulator, hSTING
ACCESSION NO.:NP_938023
PROTEIN GI NO.:38093659
GENE ID:340061
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:MPYS Antibody: MPYS is a recently identified plasma membrane tetraspanner that is associated with major histocompatibility complex class II (MHC-II) and mediates its transduction of apoptotic signals. It has also been found to be associated with VISA, a mitochondrial protein that acts as an adaptor in virus-triggered signaling. MPYS also interacts with IRF3 and recruits the kinase TBK1 to the VISA-associated complex, acting as a critical mediator of virus-triggered IRF3 activation and interferon (IFN) expression. It is thought that the binding of nucleic acid to the innate immune protein RIG-I causes complex formation between RIG-I, VISA, and MPYS. This complex then recruits TBK1 to phosphorylate IRF3 which then directly activates IFN transcription. At least three isoforms of MPYS are known to exist.
REFERENCES:1) Jin et al. Mol. Cell. Biol.2008; 28:5014-26.
2) Zhong et al. Immunity2008; 29:538-50.

ANTIBODIES FOR RESEARCH USE ONLY.

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