Datasheet

MMS21 Antibody
CATALOG NUMBER: 5759

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (80%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:MMS21 antibody can be used for detection of MMS21 by Western blot at 0.5 - 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 27 kDa

Observed: 30 kDa
IMMUNOGEN:MMS21 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human MMS21.

The immunogen is located within amino acids 170 - 220 of MMS21.
HOST SPECIES:Rabbit

Properties

PURIFICATION:MMS21 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MMS21 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MMS21 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MMS21 Antibody: NSE2, MMS21, ZMIZ7, C8orf36, E3 SUMO-protein ligase NSE2, MMS21 homolog, hMMS21
ACCESSION NO.:NP_775956
PROTEIN GI NO.:27734761
OFFICIAL SYMBOL:NSMCE2
GENE ID:286053

Background

BACKGROUND:MMS21 Antibody: MMS21, also known as NSE2, is a SUMO ligase that in combination with the SMC5/6 complex is required for the prevention of DNA damage induced apoptosis by facilitating DNA repair in human cells. MMS21-dependent sumoylation is integral and important to the cohesion mechanism and mitotic progression; this function appears to be independent of SMC6. MMS21 mediates SUMO attachment to various proteins such as SMC6L1 and TRAX, and possibly the cohesin components RAD21 and STAG2.
REFERENCES: 1) Potts PR and Yu H. Human MMS21/NSE2 is a SUMO ligase required for DNA repair. Mol. Cell. Biol. 2005; 25:7021-32.
2) Behlke-Steinert S, Touat-Todeschini L, Skoufias DA, et al. SMC5 and MMS21 are required for chromosome cohesion and mitotic progression. Cell Cycle 2009; 8:2211-8.
3) Bermudez-Lopez M, Ceschia A, de Piccoli G, et al. The Smc5/6 complex is required for dissolution of DNA-mediated sister chromatid linkages. Nucleic Acids Res. 2010; 38:6502-12.

For Research Use Only