Datasheet

MMP9 Antibody
CATALOG NUMBER: 7471

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rabbit: (100%), Bovine: (100%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:MMP9 antibody can be used for detection of MMP9 by Western blot at 1 - 2 μg/ml.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1402 - Mouse Lung Tissue Lysate
 2) Cat. No. 10-101 - Human Lung Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 76 kDa

Observed: 73kDa
SPECIFICITY:MMP9 antibody is human, mouse and rat reactive. At least three isoforms of MMP9 are known to exist; this antibody only recognizes the two longest isoforms.
IMMUNOGEN:MMP9 antibody was raised against a 16 amino acid peptide near the center of human MMP9.

The immunogen is located within amino acids 260 - 310 of MMP9 .
HOST SPECIES:Rabbit

Properties

PURIFICATION:MMP9 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MMP9 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MMP9 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MMP9 Antibody : GELB, CLG4B, MMP-9, MANDP2, Matrix metalloproteinase-9, 92 kDa gelatinase
ACCESSION NO.:NP_004985
PROTEIN GI NO.:74272287
OFFICIAL SYMBOL:MMP9
GENE ID:4318

Background

BACKGROUND:The matrix metalloproteinase (MMP) family are a family of proteins that are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases (1). MMP9 degrades type IV and V collagens (2) and studies suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow (3) and plays a role in tumor-associated tissue remodeling (4).
REFERENCES: 1) Iyer RP, Patterson NL, Fields GB, et al. The history of matrix metalloproteinases: milestones, myths, and misperceptions. Am. J. Heart Circ. Physiol 2012; 303:H919-30.
2) Murphy G, Knauper V, Atkinson S, et al. Matrix metalloproteinases in arthritic disease. Arthritis Res. 2002; 4 Suppl. 3:S39-49.
3) Sweeney EA, Lortat-Jacob H, Priestley GV, et al. Sulfated polysaccharides increase plasma levels of SDF-1 in monkeys and mice: involvement in mobilization of stem/progenitor cells. Blood 2002; 99:44-51.
4) Rhee JW, Lee KW, Sohn WJ, et al. Regulation of matrix metalloproteinase-9 gene expression and cell migration by NF-kappa B in response to CpG-oligodeoxynucleotides in RAW 264.7 cells. Mol. Immunol. 2007; 44:1393-400.

For Research Use Only