MLLT10 Antibody Cat. No.: 60-305

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: This MLLT10 (AF10) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-323 amino acids from the Central region of human MLLT10 (AF10).
TESTED APPLICATIONS: IF, WB
APPLICATIONS: For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
PREDICTED MOLECULAR WEIGHT: 113 kDa

psi-iconProperties
PURIFICATION:This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
CLONALITY:Polyclonal
ISOTYPE:Rabbit Ig
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Supplied in PBS with 0.09% (W/V) sodium azide.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:MLLT10
ALTERNATE NAMES:Protein AF-10, ALL1-fused gene from chromosome 10 protein, MLLT10, AF10
ACCESSION NO.:P55197
PROTEIN GI NO.:527504034
GENE ID:8028
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Translocations affecting chromosome 11q23 involve many partner chromosome regions and occur in various leukemias. The 11q23 gene involved in the translocations is MLL. MLLT10 is the partner gene to MLL1 involved in t(10;11)(p12;q23) translocations. In an analysis of two leukemia patients, the in t(10;11)(p12;q23) translocation fuses MLL1, a SET domain containg histone methyltransferase, to the MLLT10 gene. The MLLT10 gene encodes a predicted 1,027-amino acid protein containing an N-terminal zinc finger and a C-terminal leucine zipper domain. The MLLT10 gene is one of the few MLL partner genes to be independently rearranged with a third gene in leukemia, the CALM gene in the t(10;11)(p12;q14) translocation. Chimeric fusion proteins MLL/AF10 and CALM/AF10 consistently retain the leucine zipper motif of MLLT10. The leucine zipper interacts with GAS41, a protein previously identified as the product of an amplified gene in a glioblastoma. GAS41 interacts with integrase interactor-1 (INI1), a component of the SWI/SNF complex, which acts to remodel chromatin and to modulate transcription. Retention of the leucine zipper in the MLL and CALM fusions suggested that a key feature of these chimeric proteins may be their ability to interfere in normal gene regulation through interaction with the adenosine triphosphate-dependent chromatin remodeling complexes.
REFERENCES:1) Perrin, L., et al., Mol. Cell. Biol. 23(1):119-130 (2003).
2) Roll, P., et al., Cancer Genet. Cytogenet. 135(2):187-191 (2002).
3) Nakamura, T., et al., Mol. Cell 10(5):1119-1128 (2002).
4) Debernardi, S., et al., Blood 99(1):275-281 (2002).

ANTIBODIES FOR RESEARCH USE ONLY.

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