MIP-2 Antibody Cat. No.: XP-5149

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Rat
IMMUNOGEN: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat GROβ/MIP-2 (Rat GROβ/Macrophage Inflammatory Protein-2).
TESTED APPLICATIONS: ELISA, Neut, WB
APPLICATIONS: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat GROβ/MIP-2 (100 ng/mL), a concentration of 3.0 - 5.0 μg/mL of this antibody is required.

ELISA:

To detect Rat GROβ/MIP-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat GROβ/MIP-2.

Sandwich: To detect Rat GROβ/MIP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat GROβ/MIP-2 (XP-5149Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GROβ/MIP-2.

Western Blot:

To detect Rat GROβ/MIP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat GROβ/MIP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

psi-iconProperties
PURIFICATION:Anti-Rat GROβ/MIP-2 specific antibody was purified by affinity chromatography employing immobilized Rat GROβ/MIP-2 matrix.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Lyophilized
STORAGE CONDITIONS:GROB/MIP-2 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:Cxcl2
ALTERNATE NAMES:Mip-2, Scyb2, Cinc3, Mip-2, Mip2, C-X-C motif chemokine 2, Cytokine-induced neutrophil chemoattractant 3, CINC-3
ACCESSION NO.:P30348
PROTEIN GI NO.:266536
GENE ID:114105
USER NOTE:Centrifuge vial prior to opening.
psi-iconBackground and References
BACKGROUND:Macrophage inflammatory protein 2 (MIP-2) is a chemokine that attracts neutrophils, and its secretion from intestinal epithelial cells is enhanced by inflammatory stimuli such as interleukin 1beta.

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