Datasheet

MINA Antibody
CATALOG NUMBER: 5617

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (80%), Rat: (80%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:MINA antibody can be used for detection of MINA by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1301 - Human Heart Tissue Lysate
IMMUNOGEN:MINA antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human MINA.

The immunogen is located within amino acids 380 - 430 of MINA.
HOST SPECIES:Rabbit

Properties

PURIFICATION:MINA Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MINA Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MINA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MINA Antibody: ROX, MDIG, NO52, MINA53, Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA, 60S ribosomal protein L27a histidine hydroxylase, ROX
ACCESSION NO.:NP_694822
PROTEIN GI NO.:110227621
OFFICIAL SYMBOL:MINA
GENE ID:84864

Background

BACKGROUND:MINA Antibody: MINA is nuclear localized, myc-inducible protein that is thought to play a role in mammalian cell proliferation. Treatment of cancer cells lines such as the colon cancer cell line SW680 with siRNA against MINA inhibits cell growth, demonstrating that MINA may be a potential therapeutic target. MINA regulates several genes related to cell adhesion and metabolism that have also been shown to be regulated by c-Myc, but also regulates other genes whose expression are not modulated by c-Myc such as EGFR, IL-6 and HGF. MINA has also been found to act as a repressor to IL-4 expression in T cells, indicating that it may also play a role in T cell differentiation and genetic variation in T helper type 2 bias.
REFERENCES: 1) Tsuneoka M, Kody Y, Soejima M, et al. A novel myc target gene, mina53, that is involved in cell proliferation. J. Biol. Chem.2002; 277:35450-9.
2) Teye K, Tsuneoka M, Arima N, et al. Increased expression of a Myc target gene Mina53 in human colon cancer. Am. J. Pathol.2004; 164:205-16.
3) Komiya K, Sueoka-Aragane N, Sato A, et al. Mina53, a novel c-Myc target gene, is frequently expressed in lung cancers and exerts oncogenic property in NIH/3T3 cells. J. Cancer Res. Clin. Oncol.2010; 136:465-73.
4) Okamoto M, Van Stry M, Chung L, et al. Mina, an IL4 repressor, controls T helper type 2 bias. Nat. Immunol.2009; 10:872-9.

For Research Use Only