Datasheet

Mimitin Antibody
CATALOG NUMBER: 5445

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:Mimitin antibody can be used for detection of Mimitin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1207 - Raji Cell Lysate
 2) Cat. No. 17-007 - Raji Cell Slide
IMMUNOGEN:Mimitin antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Mimitin.

The immunogen is located within the last 50 amino acids of Mimitin.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Mimitin Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Mimitin Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Mimitin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Mimitin Antibody: MMTN, B17.2L, mimitin, NDUFA12L, Mimitin, mitochondrial, Myc-induced mitochondrial protein, MMTN
ACCESSION NO.:NP_777549
PROTEIN GI NO.:29789409
OFFICIAL SYMBOL:NDUFAF2
GENE ID:91942

Background

BACKGROUND:Mimitin Antibody: Mimitin, a small mitochondrial protein, whose transcription is directly stimulated by c-Myc, is highly expressed in 80% of esophageal squamous cell carcinomas (ESCC). Suppression of Mimitin expression by RNA interference had no effect in cancerous cell lines such as human cervical carcinoma or hepatocarcinoma cell lines, but caused a decrease in cell proliferation in human glioblastoma, embryonic lung fibroblastic cells, and ESCC, suggesting Mimitin may play a special role in these types of cells. Mimitin expression is also regulated by MAPK kinases and IL-1, but not through the NF-κB-related pathway. It will interact with the microtubular protein MAP1S and can affect the activities of caspase-3 and -7 in cells stimulated to develop apoptosis. Other experiments suggest that Mimitin also acts as a molecular chaperone for the assembly of the mitochondrial complex I.
REFERENCES: 1) Tsuneoka M, Teye K, Arima M, et al. A novel Myc-target gene, mimitin, that is involved in cell proliferation of esophageal squamous cell carcinoma. J. Biol. Chem.2005; 280:19977-85.
2) Wegrzyn P, Yarwood SJ, Fiegler N, et al. Mimitin - a novel cytokine-regulated mitochondrial protein. BMC Cell Biol.2009; 10:23.
3) Ogilvie I, Kennaway NG, and Shoubridge EA. A molecular chaperone for mitochondrial complex I assembly is mutated in a progressive encephalopathy. J. Clin. Invest.2005; 115:2784-92.

For Research Use Only